There are many methodologies in determining a current infection with Salmonella enterica serotype Dublin. These include conventional culture with serotyping or polymerase chain reaction (PCR). In addition, diagnostic laboratories in the United States and several countries in Europe also utilize an enzyme linked immunosorbent assay (ELISA) to measure the level of antibodies directed against O-antigens from Salmonella enterica serotype Dublin in both blood and milk samples. These ELISAs measure the humoral immune response as an indicator of current or previous infections. ELISA results are reported as a semi-quantitative percentage value, giving an optical density reading referable to a standard set of controls. Individual or bulk tank milk samples can also be conducted on ELISAs and have been used for screening / active surveillance programs for this serovar. Sensitivity for the serum ELISA is considerably higher than fecal culture for the identification of Salmonella enterica serotype Dublin infected cattle. As a diagnostic test, the serum ELISA is reported to perform best when used in animals between 3 and 10 months of age
Salmonella (Dublin) Serology Interpretation Guidelines
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