NOTE: Turnaround times for reporting of results may be affected by large sample numbers, availability of reagents or cell lines, confirmatory testing, legal holidays or inclement weather. Consequently, turnaround times listed for tests are estimates. There is a $10 accession fee for every submission, unless noted otherwise in the Test Information. Click on test description words for details regarding a test. If you have an urgent test request, WVDL will do the best we can to accommodate expedited testing. In this situation, an additional cost of $30 per sample will applied. It is imperative that clients call the lab (608-262-5432) immediately to request expedited testing.
NOTE: These are the cost charged to the client, but there may be additional administrative costs incurred to the owner.
Submissions for tests NOT listed here will be referred to a WVDL approved laboratory. The fee for a referral test is $25 plus the fees charged by the testing laboratory.
Test Name
Description
In State Price
Out-of-State Price
Acid Fast Stain
Stain for Mycobacteria and other Acid-fast microorganisms. For Cryptosporidia request Cryptosporidium stain. Ziehl-Neelson [LINK (10300)]
Farmed cervid head submitted for CWD Regulatory Sample Collection without an official identification
Species
Cervids
Sampling Instructions
Heads must be kept refrigerated and shipped with adequate ice packs. Heads may be frozen, but the obex sample may not be able to be collected due to physical changes during the thawing process.
Collection Container
Packing Instructions
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Tuesday, Wednesday, Thursday, Friday
Aerobic Culture
Culture on simple (BAP, EMB) bacteriologic media. Selective agar plates, rapid methods, or MALDI-TOF as needed.
10027 AEROCULT Aerobic Culture Aerobe E. coli Escherichia coli
Whole Blood or whole blood swab (may pool up to 5 samples), tonsil (may pool up to 5 samples), spleen or spleen pulp swab (may pool up to 5 samples), or lymph node (gastrohepatic and renal) NO Pooling 1 ml whole blood, ~ 5 gm tissue, swabs.
Species
Pigs
Sampling Instructions
Polyester swabs should be used for spleen or blood swab collection.
Collection Container
Whole blood (EDTA), swabs or tissues should be submitted in a labeled, sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs to arrive within 48 hours of collection.
Special Instructions
NAHLN assay. Test is performed for Foreign Animal Disease (FAD) investigations. Contact AVIC and DATCP prior to WVDL submission. Non-Negative results are confirmed at NVSL-FADDL.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
-
As needed. TAT will be based on FADI priority level.
Aleutian disease virus Real Time PCR
Detection of Aleutian disease virus by real-time PCR
30003 Mustelids Including Ferrets, Mink, Weasels, Badgers, and Otters ADV.PCR parvovirus plasmacytosis abortion mink ferret AMDV
Mustelids Including Ferrets, Mink, Weasels, Badgers, and Otters
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Amphibian/Reptile Aerobic Culture Panel
Culture on simple (BAP, EMB) bacteriologic media at temperatures appriopriate for Reptile and Amphibian organisms. Selective agar plates, rapid methods, or MALDI-TOF as needed.
Collect sample aseptically and transport in anerobic conditions. Do not add formalin or other liquids.
Collection Container
Sterile leak-proof container
Anaerobic transport media required
Packing Instructions
Label samples and ship with cold packs.
Special Instructions
Transport media can be purchased from WVDL.
WVDL Microbiology Media Order Form can be found on our website. [include link]
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Tuesday, Wednesday, Thursday, Friday
Results completed in 5-12 days.
WVDL - Barron
Monday, Tuesday, Wednesday, Thursday, Friday
Results completed in 5-12 days.
Anaplasma marginale Competitive ELISA
The Anaplasma cELISA is utilized for the detection of antibodies specific for Anaplasma in bovine serum samples. This Anaplasma cELISA kit is USDA licensed for bovine testing.
Collect serum from clotted blood in a non-additive tube (e.g. plain red top or serum separator). Excessively hemolyzed samples are not acceptable for this test.
Packing Instructions
Refrigerate, ship with cold packs overnight
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
-
Anaplasma marginale Real Time PCR
Detection of Anaplasma marginale by real-time PCR
30016 Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids ANAPLAPCR Rickettsia tick vector-borne yellow fever diarrhea ruminants
Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids
Sampling Instructions
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Aquatic Aerobic Culture and Identification
Aerobic culture on bacteriologic media for aquatic bacteria of interest
10066 Fish and other aquatic species. AEROCULTAQ AEROMONAS SALMONICIDA AQUATIC BACTERIAL IDENTIFICATION AQUATIC BACTERIAL ISOLATION CERTIFIABLE BACTERIAL PATHOGENS EDWARDSIELLA ICTALURI ENTERIC REDMOUTH DISEASE ENTERIC SEPTICEMIA OF CATFISH FURUNCULOSIS YERSINIA RUCKERI fish bacteria
This test is approved for chicken serum. Other avian serum types tested upon request, call the Barron laboratory for details (715-637-3151 or 800-771-8387)
Collection Container
Packing Instructions
Refrigerate; ship with cold packs
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Barron
-
Avian metapneumovirus Real Time PCR
Detection and differentiation of Avian metapneumovirus Type A, B and C by real-time PCR
30029 Birds Turkey Rhinotracheitis swollen head syndrome aMPV APNEUVPCR pneumovirus
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Avian paramyxovirus Type 1 Real Time PCR
Detection of avian paramyxovirus type 1 (APMV-1) by real time PCR.
Oropharyngeal swab (may pool up to 5), tracheal swab (may pool up to 5), cloacal swab (may pool up to 5), cloacal & oropharyngeal swab pool from 1 bird, tissue samples pooled from 1 bird. Pooled swabs should be submitted in 3ml (5 swabs) or in 5.5 ml (up to 11 swabs) Viral Transport Media (VTM) or Brain heart infusion broth (BHI).
Species
Birds
Sampling Instructions
Swabs should be submitted in viral transport media or BHI both. Swabs must be removed from media. Swirl each swab in the liquid, press the tip of the swab on the side of the tube to release remaining liquid.
Collection Container
BHI broth, viral transport media or swabs should be submitted in a labeled, sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs to arrive within 48 hours of collection.
Special Instructions
NAHLN assay. Subtyping may be performed if the result is non-negative. Non-negative results are confirmed at NVSL.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Tuesday, Wednesday, Thursday, Friday
As needed. Testing will be completed within 48 hrs of receipt.
Avian paramyxovirus Type 2 Hemagglutination Inhibition (HI)
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Collect sample aseptically. Do not add formalin or other liquids.
Collection Container
Sterile leak-proof container
Packing Instructions
Label samples and ship with cold packs.
Special Instructions
Swabs must be in transport media
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Barron
Monday, Tuesday, Wednesday, Thursday, Friday
Results completed in 5-12 days.
WVDL - Madison
Monday, Tuesday, Wednesday, Thursday, Friday
Results completed in 5-12 days.
Avian Susceptibility testing using MIC
Testing on Staphylococcus species isolated from milk samlpes using automated Sensititre instrumentation. Panel includes ampicillin, penicillin, erythromycin, oxacillin, pirlimycin, penicillin/novobiocin, tetracycline, cephalothin, ceftiofur and sulfadimethoxine
Innoculate single well isolated colony onto sterile media that supports target's growth.
Non selective media is reccomended (BAP, Chocolate, TSA)
Collection Container
Appropriate culture media that supports bacterial growth (culture plate/slant)
Packing Instructions
Label samples and ship with cold packs.
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Tuesday, Wednesday, Thursday, Friday
Results available in 5 to 12 days
WVDL - Barron
Tuesday, Wednesday, Thursday, Friday
Results in 5 to 12 days
Avian- Mycoplasmopsis (Mycoplasma) PCR Panel
Detection of Mycoplasmopsis (Mycoplasma) synoviae, gallispeticum and meleagridis by real-time PCR
30055 Birds MG chronic respiratory disease of chickens infectious sinusitis of turkeys avian mycoplasmpsis mycoplasma arthritis MS mycoplasma air sacculitis mycoplasma infectious stunting MM
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Bacterial Culture of Neonatal Mammalian Liquid Feeds
1-3 ml fluids: bronchoalveloar, transtracheal wash
1g tissues: lung
Species
Cattle, Goats, Sheep, Gazelle, Antelope, and Deer
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Wednesday, Friday
Results available within 24 hours of test set up.
Bluetongue virus by Agar Gel Immunodiffusion (AGID)
Agar gel immunodiffusion is utilizied for the detection of BTV antibodies and is often required for import or export of cattle or bovine. BTV and EHD have known cross-reactivity on AGIDs. Susceptible species: sheep, cattle, ruminant animals. (Bovine, Bovine other, Caprine, Ovine)
20262 Cattle BTV Bluetongue Agar Gel Immunodiffusion Assay AGID
1-3 mL serum or 3-5 mLs clotted blood. If requesting 3 or more serological assays, please submit 10 mLs serum to accommodate all tests requested.
Species
Cattle
Sampling Instructions
Collection Container
Collect serum from clotted blood in a non-additive tube (e.g. plain red top or serum separator). Excessively hemolyzed samples are not acceptable for this test.
Packing Instructions
Refrigerate, ship with cold packs overnight
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
-
Bluetongue Virus Competitive ELISA
The BTV cELISA is utilized for the detection of BTV antibodies in bovine sera. This kit manufacturer has validated this test for serum/plasma from bovine, ovine, caprine and deer species. Non-bovine species and plasma can be tested but is not covered under the WVDL's standard operating procedures. Non-bovine specimens will be reported with a disclaimer.
20275 Cattle. Goats and Sheep can be ran with disclaimer. BTV Bluetongue
1-3 mL serum or 3-5 mLs clotted blood. If requesting 3 or more serological assays, please submit 10 mLs serum to accommodate all tests requested.
Species
Cattle. Goats and Sheep can be ran with disclaimer.
Sampling Instructions
Collection Container
Collect serum from clotted blood in a non-additive tube (e.g. plain red top or serum separator). Excessively hemolyzed samples are not acceptable for this test.
Packing Instructions
Refrigerate, ship with cold packs overnight
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
-
Bluetongue virus Real Time PCR (Semen)
Detection of Bluetongue virus by real-time PCR in semen samples
Semen (200uL Extended semen: 4 x 250cc or 2 x 500cc straws)
Species
Cattle
Sampling Instructions
Semen samples should be sent frozen in liquid nitrogen.
Collection Container
Leak-proof tubes or straws.
Packing Instructions
Please utilize a semen tank map and place semen into numerically identified holders that correspond to the paperwork. Bundle semen straws for PCR separately from semen straws for virus isolation or culture testing. Using separate semen tanks for each section is ideal.
Special Instructions
This test is specific for semen samples. We cannot pool semen straws from different collection dates.
1-3 ml swabs: conjunctival, nasal, oral/pharyngeal, tracheal
1g tissues: liver, lung
Species
Cats, Dogs
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Bovine Abortion PCR Panel
Detection of pathogens commonly attributed to bovine abortion; includes Bovine viral diarrhea virus (BVDV), Bovine herpes virus Type 1 (BHV-1), Leptospira spp, Neospora caninum, Ureaplasma diversum by real-time PCR
30172 Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids ABORTPNLBOV IBR BHV-1 LEPTO NEO UREA fetus fetal BVDV
1g tissues: lung, liver, placenta and fetal tissue
Species
Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Wednesday, Friday
Results available within 24 hours of test set up.
Bovine adenovirus Type 3 Real Time PCR
Detection of Bovine adenovirus Type 3 by real-time PCR
30185 Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids BADENO3PCR BAV-3 BAdV-3 Adenovirus
Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Wednesday, Friday
Results available within 24 hours of test set up.
Bovine adenovirus Type 5 Real Time PCR
Dectection of Bovine adenovirus Type 5 by real-time PCR
30198 Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids BADENO5PCR BAV-5 BAdV-5 Adenovirus weak calf syndrome.
Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Wednesday, Friday
Results available within 24 hours of test set up.
Bovine Bacterial Respiratory PCR Panel
Detection of bacterial pathogens commonly attributed to respiratory disease in bovine; includes Histophilus somni, Mannheimia haemolytica, Pasteurella multocida, Mycoplasma bovis, and Bibersteinia treholosi by real-time PCR
30211 Bovids, including Cattle, Sheep, Goats, Antelope, and Gazelle BRESPNLPCR H. somni Mannheimia pasturella bibersteinia mycoplasma
Bovids, including Cattle, Sheep, Goats, Antelope, and Gazelle
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Wednesday, Friday
Results available within 24 hours of test set up.
Bovine coronavirus Real Time PCR
Detection of Bovine coronavirus real-time PCR
30224 Cattle, Sheep, Goats, Deer, and Camelids including Llamas and Alpacas CORONA.PCR respiratory enteric winter dysentery BRDC diarrhea calves BCoV
Cattle, Sheep, Goats, Deer, and Camelids including Llamas and Alpacas
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Bovine coronavirus assay will detect both the respiratory and enteric strains of coronavirus in bovines. Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Wednesday, Friday
Results available within 24 hours of test set up.
Bovine Enteric Adult PCR Panel
Detection of the most common viral, bacterial and protozoal causes of diarrhea in juvenile/adult animals; includes Bovine coronavirus (BCoV), and Salmonella spp by real-time PCR
30237 Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids ENTPNLADLT diarrhea winter dysentery coronavirus salmonella
Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids
Sampling Instructions
Bovine coronavirus assay will detect both the respiratory and enteric strains of coronavirus in bovines.
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types.
Contact the lab for more information.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Wednesday, Friday
Results available within 24 hours of test set up.
Bovine Group Abortion Culture Panel
Culture for aerobic and microaerphilic bacteria known to cause abortions including Listeria, Campylobacter, and Salmonella
Mycoplasmopsis and Ureaplamsa not included.
Bovine Group includes cattle, goat, sheep, deer, alpaca, llama, and camel
Cattle, Goat, Sheep, Deer, Alpaca, Llama, and Camel
Sampling Instructions
Collect sample aseptically. Do not add formalin or other liquids.
Collection Container
Sterile, sealed, leak-proof container
Packing Instructions
Label samples and ship with cold packs.
Special Instructions
Swabs must be in transport media
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Barron
Monday, Tuesday, Wednesday, Thursday, Friday
Results completed in 5-12 days.
WVDL - Madison
Monday, Tuesday, Wednesday, Thursday, Friday
Results completed in 5-12 days.
Bovine Group Abortion Culture Panel w Fungal Screen
Culture for aerobic and microaerphilic bacteria known to cause abortions including Listeria, Campylobacter, Salmonella, and fungal culture.
Mycoplasmopsis and Ureaplamsa not included.
Bovine Group includes cattle, goat, sheep, deer, alpaca, llama, and camel
11080 Bovids, Cervids and Camelids including Cattle, Sheep, Goats, Llamas, Alpaca, and Deer ABORCULT Abortion Fetus Fetal Tissue Listeria Campylobacter Salmonella Fungal Fungus
Cattle, Goats, Sheep, Gazelle, Antelope, other Bovids, and Deer
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Wednesday, Friday
Results available within 24 hours of test set up.
Bovine herpesvirus Type 1 Real Time PCR (Semen)
Detection of Bovine herpesvirus Type 1 (IBR) by real-time PCR in semen samples
Semen (200uL Extended semen: 4 x 250cc or 2 x 500cc straws)
Species
Cattle
Sampling Instructions
Semen samples should be sent frozen in liquid nitrogen.
Collection Container
Leak-proof tubes or straws.
Packing Instructions
Please utilize a semen tank map and place semen into numerically identified holders that correspond to the paperwork. Bundle semen straws for PCR separately from semen straws for virus isolation or culture testing. Using separate semen tanks for each section is ideal.
Special Instructions
This test is specific for semen samples. We cannot pool semen straws from different collection dates.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Tuesday
Results available within 24 hours of test set up.
Bovine herpesvirus Type 1 Serum Neutralization (OIE Method)
Detection of antibodies to bovine herpesvirus 1 (BHV-1), the causative agent of infectious bovine rhinotrachetitis (IBR) virus by OIE serum neutralization method.
Collect serum from clotted blood in a non-additive tube. Avoid hemolysis.
Collection Container
Serum samples should be submitted in serum separator tubes, uncoated/plain tubes may be appropriate for spun serum.
Packing Instructions
Label all samples with unique identifiers. Ship overnight on cold packs.
Special Instructions
OIE approved method for detection of neutralizing antibodies. Submit paired (acute & convalescent) serum samples together. Convalescent samples should be collected 10 to 14 days after the acute animal has been sampled. Separate acute serum from clot and store frozen until the convalescent sample has been collected. Comparison of sera from 2 points in time requires testing on the same day.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Thursday
Results available in 4 to 5 days.
Bovine herpesvirus Type 1 Serum Neutralization (OIE Method) (titered to endpoint)
Detection of antibodies to bovine herpesvirus 1 (BHV-1), the causative agent of infectious bovine rhinotrachetitis (IBR) virus by OIE serum neutralization method. Serum is titered to endpoint.
Collect serum from clotted blood in a non-additive tube. Avoid hemolysis.
Collection Container
Serum samples should be submitted in serum separator tubes, uncoated/plain tubes may be appropriate for spun serum.
Packing Instructions
Label all samples with unique identifiers. Ship overnight on cold packs.
Special Instructions
OIE approved method for detection of neutralizing antibodies. Submit paired (acute & convalescent) serum samples together. Convalescent samples should be collected 10 to 14 days after the acute animal has been sampled. Separate acute serum from clot and store frozen until the convalescent sample has been collected. Comparison of sera from 2 points in time requires testing on the same day.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Thursday
Results available in 4 to 5 days
Bovine herpesvirus Type 1 Serum Neutralization (titered to endpoint)
Detection of antibodies to bovine herpesvirus 1 (BHV-1), the causative agent of infectious bovine rhinotrachetitis (IBR) virus by serum neutralization. Serum are titered to endpoint.
Collect serum from clotted blood in a non-additive tube. Avoid hemolysis.
Collection Container
Serum samples should be submitted in serum separator tubes, uncoated/plain tubes may be appropriate for spun serum.
Packing Instructions
Label all samples with unique identifiers. Ship overnight on cold packs.
Special Instructions
Submit paired (acute & convalescent) serum samples together. Convalescent samples should be collected 10 to 14 days after the acute animal has been sampled. Separate acute serum from clot and store frozen until the convalescent sample has been collected. Comparison of sera from 2 points in time requires testing on the same day.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Friday
Results available in 3 days
Bovine herpesvirus Type 2 Real Time PCR
Detection of Bovine herpesvirus Type 2 by real-time PCR
30276 Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids BHV2PCR BHV-2 mammalitis teat udder lesion
Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids
Sampling Instructions
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
If an FAD (Foreign Animal Disease) differential is suspected, contact the AVIC and DATCP prior to WVDL submission.
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types.
Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Bovine herpesvirus Type 4 Real Time PCR
Detection of Bovine herpesvirus Type 4 by real-time PCR
30289 Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids BHV4PCR BHV-4 abortion respiratory reproductive lesion
Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
If an FAD (Foreign Animal Disease) differential is suspected, contact the AVIC and DATCP prior to WVDL submission.
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types.
Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Bovine immunoglobulin (IgG) Quantification by Radial Immunodiffusion
Serum, plasma, whole blood, colostrum, 1 ml minimum* *If colostrum is also being submitted for other tests, please submit 15 ml minimum.
Species
Cattle
Sampling Instructions
Collection Container
Packing Instructions
Refrigerate; ship with cold packs
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Barron
-
Bovine leukemia virus by Agar Gel Immunodiffusion (AGID)
Agar gel immunodiffusion is utilizied for the detection of BLV antibodies and is often required for import or export of cattle or bovine. Susceptible species: sheep, cattle, ruminant animals. (Bovine, Bovine other, Caprine, Ovine)
1-3 mL serum or 3-5 mLs clotted blood. If requesting 3 or more serological assays, please submit 10 mLs serum to accommodate all tests requested.
Species
Cattle
Sampling Instructions
Collection Container
Collect serum from clotted blood in a non-additive tube (e.g. plain red top or serum separator). Excessively hemolyzed samples are not acceptable for this test.
Packing Instructions
Refrigerate, ship with cold packs overnight
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
-
Bovine leukemia virus Competitive ELISA
The BLV cELISA is utilized for the detection of BLV antibodies in bovine sera. This kit manufacturer has validated this test for bovine serum/plasma only. However, plasma, buffalo serum/plasma will be reported with a disclaimer.
1-3 mL serum or 3-5 mLs clotted blood. If requesting 3 or more serological assays, please submit 10 mLs serum to accommodate all tests requested.
Species
Cattle
Sampling Instructions
Collection Container
Collect serum from clotted blood in a non-additive tube (e.g. plain red top or serum separator). Excessively hemolyzed samples are not acceptable for this test.
Packing Instructions
Refrigerate, ship with cold packs overnight
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
-
Bovine leukemia virus Real Time PCR
Detection of Bovine leukemia virus by real-time PCR
30302 Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids BLV enzootic bovine leucosis Hodgkin lymphoma non-Hodgkin
Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids
Sampling Instructions
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Bovine leukemia virus Real Time PCR (Semen)
Detection of Bovine leukemia virus by real-time PCR in semen
Semen (200uL Extended semen: 4 x 250cc or 2 x 500cc straws)
Species
Cattle
Sampling Instructions
Semen samples should be sent frozen in liquid nitrogen.
Collection Container
Leak-proof tubes or straws.
Packing Instructions
Please utilize a semen tank map and place semen into numerically identified holders that correspond to the paperwork. Bundle semen straws for PCR separately from semen straws for virus isolation or culture testing. Using separate semen tanks for each section is ideal.
Special Instructions
This test is specific for semen samples. We cannot pool semen straws from different collection dates.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Tuesday
Results available within 24 hours of test set up.
Bovine Oral Lesions Panel
Detection of pathogens that mimic Foot and Mouth disease in bovine; includes Bovine viral diarrhea virus (BVDV), Bovine herpesvirus Type 1 (BHV-1), Bovine herpesvirus Type 2 (BHV-2), Bovine herpesvirus Type 4 (BHV-4), Bluetongue virus (BTV), Epizootic hemorrhagic disease virus (EHD), Contagious ecthyma (ORF), Ovine herpesviurs Type 2 (OvHV-2, Malignant catarrhal fever (MCF)), and Bovine papular stomatitis virus (BPSV) by real time PCR.
30328 Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids MIMICPNLBOV BVDV IBR BHV-1 BPSV BHV-2 BHV-4 MCF ORF BTV EHD
Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
If an FAD (Foreign Animal Disease) differential is suspected, contact the AVIC and DATCP prior to WVDL submission.
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types.
Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Bovine Pink Eye PCR Panel
Detection of pathogens commonly attributed to pink eye (conjunctivitis) in bovine; includes Bovine herpes virus Type 1 (BHV-1), Mycoplasma bovis, Moraxella bovis, Moraxella bovoculi, and Mycoplasma bovoculi by real-time PCR
30367 Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids PINKPNLPCR conjuctival IBK New Forest Eye keratoconjuctivitis eye
Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Bovine Respiratory Panel 2
Detection of Bovine parainfluenza type 3 and Influenza D viruses by real-time PCR
31108 Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids
Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids
Sampling Instructions
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
-
Bovine Respiratory PCR Panel
Detection of pathogens commonly attributed to respiratory disease in bovine; includes Bovine viral diarrhea virus (BVDV), Bovine herpes virus Type 1 (BHV-1), Bovine respiratory syncytial virus (BRSV), Bovine coronavirus (BCoV), Histophilus somni, Mannheimia haemolytica, Pasteurella multocida, Mycoplasma bovis, and Bibersteinia treholosi, by real-time PCR
Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Wednesday, Friday
Results available within 24 hours of test set up.
Bovine respiratory syncytial virus Real Time PCR
Bovine respiratory syncytial virus by real-time PCR
30393 Cattle, Goats, Sheep, Gazelle, Antelope, other Bovids, and Deer BRSVPCR respiratory pneumonia BRD
2-3 ml fluids: bronchoalveloar fluid, transtracheal wash
1g tissues: lung
Species
Cattle, Goats, Sheep, Gazelle, Antelope, other Bovids, and Deer
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Wednesday, Friday
Results available within 24 hours of test set up.
Bovine rotavirus Group A Real Time PCR
Detction of Bovine rotavirus Group A by real-time PCR
30419 Cattle, Goats, Sheep, Gazelle, Antelope, other Bovids, and Deer ROTA.PCR diarrhea neonatal calves
Cattle, Goats, Sheep, Gazelle, Antelope, other Bovids, and Deer
Sampling Instructions
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Wednesday, Friday
Results available within 24 hours of test set up.
Bovine Scours A PCR Panel
Detection of the most common viral, bacterial and protozoal causes of diarrhea; includes Bovine coronavirus (BCoV), Bovine rotavirus Group A (BRV), and Cryptosporidium parvum by real-time PCR.
30432 Cattle, Goats, Sheep, Gazelle, Antelope, other Bovids, and Deer SCOURPNLA diarrhea viral protozoa
Cattle, Goats, Sheep, Gazelle, Antelope, other Bovids, and Deer
Sampling Instructions
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Wednesday, Friday
Results available within 24 hours of test set up.
Bovine Scours B PCR Panel
Detection of the most common viral, bacterial and protozoal causes of diarrhea in calves greater than 7 days of age; includes Bovine coronavirus (BCoV), Bovine rotavirus Group A (BRV), Cryptosporidium parvum, and Salmonella spp by real-time PCR
30445 Cattle, Goats, Sheep, Gazelle, Antelope, other Bovids, and Deer SCOURPNLB diarrhea viral bacterial protozoa calves young
Cattle, Goats, Sheep, Gazelle, Antelope, other Bovids, and Deer
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Wednesday, Friday
Results available within 24 hours of test set up.
Bovine Scours C PCR Panel
Detection of the most common viral, bacterial and protozoal causes of diarrhea in calves less than equal to 7 days of age; includes Bovine coronavirus (BCoV), Bovine rotavirus Group A (BRV), Cryptosporidium parvum, Salmonella spp, and Escherichia coli K99 (adhesin) and Intimin proteins by real-time PCR
30458 Cattle, Goats, Sheep, Gazelle, Antelope, other Bovids, and Deer SCOURPNLC diarrhea viral bacterial protozoa calves young
Cattle, Goats, Sheep, Gazelle, Antelope, other Bovids, and Deer
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Wednesday, Friday
Results available within 24 hours of test set up.
Bovine Teat Lesion Panel
Detection of Bovine herpesvirus type 2 and Bovine herpesvirus type 4 by real-time PCR
31446 Bovids including Cattle, Sheep, Goats, Antelope, and Gazelle BHV-2, BHV-4
Bovids including Cattle, Sheep, Goats, Antelope, and Gazelle
Sampling Instructions
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
***If a FAD (Foreign Animal Disease) differential is suspected, contact the AVIC and DATCP prior to WVDL submission.*** Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up
Bovine torovirus (Breda virus) Real Time PCR
Detection of Bovine torovirus (Breda virus) by real-time PCR
30471 Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids BREDAPCR coronavirus ungulates enteric
Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
-
As Needed.
Bovine viral diarrhea virus Antigen Capture ELISA
The BVD ACE is utilized for the detction of antigen in either serum or ear notch biopsy samples from clinically or persistently infected animals. Animals must be 3 months of age or older.
1-3 mL serum or 3-5 mLs clotted blood. If requesting 3 or more serological assays, please submit 10 mLs serum to accommodate all tests requested.
Species
Cattle
Sampling Instructions
Collection Container
Collect serum from clotted blood in a non-additive tube (e.g. plain red top or serum separator). Excessively hemolyzed samples are not acceptable for this test.
Packing Instructions
Refrigerate, ship with cold packs overnight
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
-
Bovine viral diarrhea virus P80 Antibody ELISA
Detection of antibodies to bovine viral diarrhea virus (BVDV) by ELISA
1-3ml of fluids Swab in 1-3 ml media (VTM, BHI, PBS) 1 g fresh tissue Ear notch: dime sized piece of tissue
Species
Cattle, Goats, Sheep, Gazelle, Antelope, other Bovids, and Camelids including Llamas and Alpacas
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab. Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing. Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Submit whole blood samples for animals <9 weeks of age. Animals must be at least 9 weeks old to use serum for testing.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hours of test set up.
Bovine viral diarrhea virus Real Time PCR (Pool)
Detection of Bovine viral diarrhea virus by real-time PCR in pooled samples
30523 Cattle, Goats, Sheep, Gazelle, Antelope, other Bovids, and Camelids including Llamas and Alpacas BVDPCRPL BVDSWPL mucosal disease pestivirus export regulatory BVDV
1-3ml of fluids Swab in 1-3 ml media (VTM, BHI, PBS) 1 g fresh tissue Ear notch: dime sized piece of tissue
Species
Cattle, Goats, Sheep, Gazelle, Antelope, other Bovids, and Camelids including Llamas and Alpacas
Sampling Instructions
Ear notches (EN): Can pool up to 24 ear notches. An EN from any age animal may be submitted.
1-3 ml whole blood (EDTA): Can pool up to 24 samples. Submit EDTA for animals <9 weeks of age.
2-3 ml serum: Can pool up to 24 samples. Animals must be at least 9 weeks old to use serum for testing.
Camelid serum or whole blood samples are pooled in groups up to 5.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Screening pools can significantly reduce testing costs without compromising test sensitivity. If the pool test yields a positive result, the samples comprising that pool will be tested individually by BVDv Real Time-PCR at an additional cost. Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hours of test set up.
Bovine viral diarrhea virus Real Time PCR (Semen)
Detection of Bovine viral diarrhea virus by real-time PCR in semen
Semen (200uL Extended semen: 4 x 250cc or 2 x 500cc straws)
Species
Cattle
Sampling Instructions
Semen samples should be sent frozen in liquid nitrogen.
Collection Container
Leak-proof tubes or straws.
Packing Instructions
Please utilize a semen tank map and place semen into numerically identified holders that correspond to the paperwork. Bundle semen straws for PCR separately from semen straws for virus isolation or culture testing. Using separate semen tanks for each section is ideal.
Special Instructions
This test is specific for semen samples. We cannot pool semen straws from different collection dates.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Tuesday
Results available within 24 hours of test set up.
Bovine viral diarrhea virus Type 1 & Type 2 Serum Neutralization
Detection of antibodies to bovine viral diarrhea virus (BVDV) Type 1 & Type 2 by serum neutralization.
40108 Cattle BOVINE VIRUS DIARRHEA BVD BVDV BVD SN T1 BVD SN T2 MUCOSAL DISEASE BVD1&2SN
Collect serum from clotted blood in a non-additive tube. Avoid hemolysis.
Collection Container
Serum samples should be submitted in serum separator tubes, uncoated/plain tubes may be appropriate for spun serum.
Packing Instructions
Label all samples with unique identifiers. Ship overnight on cold packs.
Special Instructions
Submit paired (acute & convalescent) serum samples together. Convalescent samples should be collected 10 to 14 days after the acute animal has been sampled. Separate acute serum from clot and store frozen until the convalescent sample has been collected. Comparison of sera from 2 points in time requires testing on the same day.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Thursday
Results available in 4 days.
Bovine viral diarrhea virus Type 1 & Type 2 Serum Neutralization (titered to endpoint)
Detection of antibodies to bovine viral diarrhea virus (BVDV) Type 1 & Type 2 by serum neutralization. Serum is titered to endpoint.
40121 Cattle BOVINE VIRUS DIARRHEA BVD BVDV BVD SN T1 BVD SN T2 MUCOSAL DISEASE
Collect serum from clotted blood in a non-additive tube. Avoid hemolysis.
Collection Container
Serum samples should be submitted in serum separator tubes, uncoated/plain tubes may be appropriate for spun serum.
Packing Instructions
Label all samples with unique identifiers. Ship overnight on cold packs.
Special Instructions
Submit paired (acute & convalescent) serum samples together. Convalescent samples should be collected 10 to 14 days after the acute animal has been sampled. Separate acute serum from clot and store frozen until the convalescent sample has been collected. Comparison of sera from 2 points in time requires testing on the same day.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Thursday
Results available in 4 days.
Bovine Viral Respiratory PCR Panel
Detection of viral pathogens commonly attributed to respiratory disease in bovine; includes Bovine viral diarrhea virus (BVDV), Bovine herpes virus Type 1 (BHV-1), Bovine respiratory syncytial virus (BRSV), and Bovine coronavirus (BCoV) by real-time PCR
30562 Cattle, Goats, Sheep, Gazelle, Antelope, other Bovids, and Deer VRESPNLPCR IBR BRSV BCoV BVDV
Cattle, Goats, Sheep, Gazelle, Antelope, other Bovids, and Deer
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Wednesday, Friday
Results available within 24 hours of test set up.
Bovine/Porcine Susceptibility testing using MIC
Testing on bacterial isolates from milk samlpes using automated Sensititre instrumentation. Panel includes ampicillin, penicillin, erythromycin, oxacillin, pirlimycin, penicillin/novobiocin, tetracycline, cephalothin, ceftiofur and sulfadimethoxine
10885 Pigs, Bovidae, Cervidae, and Camelidae Family including Cattle, Sheep, Goats, Llamas, Alpaca, Deer, Gazelle and Antelope SUSCEPTMIC KIRBY BAUER MINIMUM INHIBITORY CONCENTRATION (MIC) SENSITIVITY SUSCEPTIBILITY BY SENSITITRE
Fresh feces, intestinal contents, tissues, or swab in transport media
Species
Birds, Pigs, and Bovids including Cattle, Goats, Sheep, and Antelopes.
Sampling Instructions
Collect sample aseptically. Do not add formalin or other liquids.
Collection Container
Sterile, sealed, leak-proof container
Packing Instructions
Label samples and ship tissue/feces with cold packs. Ship Weybridge Transport Media at room temperature.
Special Instructions
Swabs must be in transport media
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Barron
Monday, Tuesday, Wednesday, Thursday, Friday
Results completed in 5-12 days.
WVDL - Madison
Monday, Tuesday, Wednesday, Thursday, Friday
Results completed in 5-12 days.
Brucella Antibody Detection by Buffered Acidified Plate Antigen (BAPA)
The buffered acidified plate antigen agglutination (BAPA) test is utilized to detect antibody against Brucella abortus and suis in bovine and cervide sera. This test is also known as Brucellosis Buffered (Acidified) Antigen Test (BBAT) or Brucellosis Buffered Plate Antigen Test (BPAT).
20210 Ruminants including Cattle, Deer, Goats, Sheep, Giraffes, and Antelope Brucella Brucellosis BAPA BBAT BPAT Bruc
Serum;1 mL ; If multiple tests are required, then a 1-2 day delay should be expected. Sending in additional sera samples will remove that delay.
Species
Ruminants including Cattle, Deer, Goats, Sheep, Giraffes, and Antelope
Sampling Instructions
Collection Container
Collect serum from clotted blood in a non-additive tube (e.g. plain red top or serum separator). Excessively hemolyzed samples are not acceptable for this test.
Packing Instructions
Refrigerate, ship with cold packs overnight
Special Instructions
Samples showing a positive reaction will be confirmed using the Brucellosis FPA.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
-
Brucella Card Agglutination
The Brucellosis Card test (Rose Bengal) is utilized to screen for antibody against Brucella abortus. There are two choices for antigen: 10-S and 150-AMP for most occasions, and 10-G which is for small ruminants (ovine and caprine). Both antigens will cross react with B. abortus, B. suis and B. melitensis.
20223 Ruminants including Cattle, Deer, Goats, Sheep, Giraffes, and Antelope Brucella Brucellosis Card Abortus Melintensis BANGS Bruc
Serum;1 mL ; If multiple tests are required, then a 1-2 day delay should be expected. Sending in additional sera samples will remove that delay.
Species
Ruminants including Cattle, Deer, Goats, Sheep, Giraffes, and Antelope
Sampling Instructions
Collection Container
Collect serum from clotted blood in a non-additive tube (e.g. plain red top or serum separator). Excessively hemolyzed samples are not acceptable for this test.
Packing Instructions
Refrigerate, ship with cold packs overnight
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
-
Brucella Fluorescent Polarization (FPA) Assay
The Brucella Fuorescence Polarization assay (FPA) is utilized to detect antibodies to Brucella abortus/suis in serum. Tests for Brucella abortus cross react with B. Suis and B. Melintensis.
20236 Ruminants including Cattle, Deer, Goats, Sheep, Giraffes, and Antelope Brucella Brucellosis FPA Abortus Bruc
Serum;1 mL ; If multiple tests are required, then a 1-2 day delay should be expected. Sending in additional sera samples will remove that delay.
Species
Ruminants including Cattle, Deer, Goats, Sheep, Giraffes, and Antelope
Sampling Instructions
Collection Container
Collect serum from clotted blood in a non-additive tube (e.g. plain red top or serum separator). Excessively hemolyzed samples are not acceptable for this test.
Packing Instructions
Refrigerate, ship with cold packs overnight
Special Instructions
Samples showing a positive reaction will be confirmed using the Brucellosis STD Tube (BTT).
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
-
Brucella Standard Tube Test (STT)
The Brucella Standard Tube Test (BTT) is utilized to detect antibody levels against Brucella abortus. Tests for Brucella abortus cross react with B. suis and B.melintensis.
20249 Ruminants including Cattle, Deer, Goats, Sheep, Giraffes, and Antelope Brucella Brucellosis BTT Tube Standard Bruc
Serum;1 mL ; If multiple tests are required, then a 1-2 day delay should be expected. Sending in additional sera samples will remove that delay.
Species
Ruminants including Cattle, Deer, Goats, Sheep, Giraffes, and Antelope
Sampling Instructions
Collection Container
Collect serum from clotted blood in a non-additive tube (e.g. plain red top or serum separator). Excessively hemolyzed samples are not acceptable for this test.
Frozen Semen. Minimum of 4 straws per collection; 0.5ml non-extended semen
Species
Cattle
Sampling Instructions
Collection date and clearly labeled EDTA or heparin blood tubes or semen straws in liquid nitrogen.
Collection Container
Packing Instructions
Special Instructions
Testing is performed at NVSL and takes an estimated 22 business days to complete. Please see their website for updated testing days of the week and turn around time.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
-
WVDL - Barron
-
Buffy Coat Virus Isolation- Bovine viral diarrhea virus confirmed by PCR
Isolation and identification of bovine viral diarrhea viurs (BVDV) from a buffy coat sample using cell culture and BVD PCR confirmation.
Sample must be received at WVDL within 7 days of bleed date.
Collection Container
Samples must be submitted in EDTA tubes.
Packing Instructions
Label all samples with unique identifiers. Ship overnight on cold packs.
Special Instructions
A buffy coat sample is required from calves < 6 months of age because of the presence of maternal antibodies in serum. Turn-around time will be affected by a drastic increase in requests for an individual test, arrival time of the specimen at the laboratory and poor quality samples that will require additional processing. Advanced scheduling is REQUIRED when submitting greater than 20 samples at one time. Please contact the Virology Section at least one week prior to submission.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Wednesday, Friday
Results complete within 10-14 Days
Camelid Abortion PCR Panel
Detection of pathogens commonly attributed to abortion in camelids; includes Bovine viral diarrhea virus (BVDV), Equine herpesvirus Type 1 (EHV-1), Leptospira spp and Neospora caninum by real-time PCR
30575 Cattle, Goats, Sheep, Gazelle, Antelope, other Bovids, and Camelids including Llamas and Alpacas ABORPNLCAM llama alpaca
Cattle, Goats, Sheep, Gazelle, Antelope, other Bovids, and Camelids including Llamas and Alpacas
Sampling Instructions
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Package placenta separately from other tissues.
Label all samples.
Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Camelid Respiratory PCR Panel
Detection of pathogens commonly attributed to respiratory disease in camelids; includes Bovine viral diarrhea virus (BVDV), Bovine respiratory syncytial virus (BRSV) and Chlamydia spp by real-time PCR
30588 Cattle, Goats, Sheep, Gazelle, Antelope, other Bovids, and Camelids including Llamas and Alpacas RESPNLCAM llama alpaca
Cattle, Goats, Sheep, Gazelle, Antelope, other Bovids, and Camelids including Llamas and Alpacas
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Campylobacter fetus fetus or C. fetus venerealis Culture
Bacterial culture of microaerophilic organisms on media selective for Campylobacter fetus
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Campylobacter jejuni/coli Culture
Bacterial culture of microaerophilic organisms on media selective for Campylobacter jejuni/coli
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Canine coronavirus Type 1 Real Time PCR
Detection of Canine coronavirus Type 1 by real-time PCR
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Canine distemper virus Real Time PCR
Detection of Canine distemper virus by real-time PCR
30653 Dogs and Mustelids including Weasels, Ferrets, and Mink. CDV.PCR footpad disease enteric respiratory
Dogs and Mustelids including Weasels, Ferrets, and Mink.
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Canine Enteric PCR Panel
Detection of pathogens commonly attributed to enteric disease in canines; includes Canine adenovirus (CAV), Canine coronavirus Type 1 (CCoV-1), and Canine parvovirus Type 2 (CPV-2) by real-time PCR
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Canine Leptospira Antibody Microagglutination Test (LeptoMAT)
The Small Animal Leptospirosis Test Serovars included are Bratislava, Canicola, Grippotyphosa, Icterohaemorrhagiae, Pomona, Autumnalis. Species can include canine, feline and exotic. Serologic diagnosis requires detection of a significant rise in antibody titer in paired serum samples 10-14 days apart.
Serum;1 mL minimum. If multiple tests are required, then a 1-2 day delay should be expected. Sending in additional sera samples will remove that delay.
Species
Mammals
Sampling Instructions
Collection Container
Collect serum from clotted blood in a non-additive tube (e.g. plain red top or serum separator). Excessively hemolyzed samples are not acceptable for this test.
Packing Instructions
Refrigerate, ship with cold packs overnight
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
-
Canine parainfluenza virus Real Time PCR
Detection of Canine parainfluenza virus by real-time PCR
1-3 ml swabs: conjunctival, nasal, oral/pharyngeal, tracheal
1g tissues: intestine, lung
Species
Dogs
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Canine parvovirus Type 2 Real Time PCR
Detection of Canine parvovirus Type 2 by real-time PCR
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Canine respiratory coronavirus Real Time PCR
Detection of Canine respiratory coronavirus by real-time PCR
1-3 ml swabs: conjunctival, nasal, oral/pharyngeal, tracheal
1g tissues: lung
Species
Dogs
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Canine Respiratory PCR Panel
Detection of pathogens commonly attributed to respiratory disease in canines; includes Canine adenovirus (CAV), Canine distemper virus (CDV), Canine herpesvirus (CHV), Canine parainfluenza virus (CPIV), Canine respiratory coronavirus (CRCoV), Influenza A virus (Flu-A), Bordetella bronchiseptica, Streptococcus equi zooepidemicus and Mycoplasma cynos by real-time PCR
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Canine/Cervid Enteric Culture Panel
Culture for enteric bacteria including Salmonella, E. coli, Yersinia, and Campylobacter jejuni/coli
10261 Dogs, Deer ENTERICULT enteric culture dog deer cervid canine K9 Campylobacter jejuni Campylobacter coli Yersinia species Salmonella species Yersinia pseudotuberculosis
Collect sample aseptically. Do not add formalin or other liquids.
Collection Container
Sterile, sealed, leak-proof container
Packing Instructions
Label samples and ship with cold packs.
Special Instructions
Swabs must be in transport media
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Barron
Monday, Tuesday, Wednesday, Thursday, Friday
Results completed in 5-12 days.
WVDL - Madison
Monday, Tuesday, Wednesday, Thursday, Friday
Results completed in 5-12 days.
Caseous Lymphadenitis ELISA
Detection of IgG antibodies specific for agent of Caseous Lymphadenitis (CLA) in sheep or goat sera. CLA is a bacterial disease of sheep and goats caused by Corynebacterium pseudotuberculosis.
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Classical swine fever virus Real Time PCR
Detection of classical swine fever (CSF) virus by real time PCR.
Whole Blood or whole blood swab (may pool up to 5 samples), tonsil or tonsil scrapping in tris-buffered tryptose broth (TBTB), spleen or spleen pulp swab, lymph node (mesenteric, submandibular, retropharyngeal) 1 ml whole blood, ~ 5 gm tissue, swabs.
Species
Pigs
Sampling Instructions
Polyester swabs should be used for spleen or blood swab collection.
Collection Container
Whole blood (EDTA), swabs or tissues should be submitted in a labeled, sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs to arrive within 48 hours of collection.
Special Instructions
NAHLN assay. Test is performed for Foreign Animal Disease (FAD) investigations. Contact AVIC and DATCP prior to WVDL submission. Non-Negative results are confirmed at NVSL-FADDL.
Cattle, Goats, Sheep, Gazelle, Antelope, other Bovids, and Horses
Sampling Instructions
Samples may be submitted to the lab for culture prior to toxin typing.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable.
A disclaimer will be included on the final report for non-validated species/specimen types.
Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Wednesday
Results available within 24 hours of test set up.
Clostridium perfringens Culture
Culture for Clostridium perfringens
10287 C.PERF.CUL Clostridium perfringens C. perfringens C. perf
Fresh feces, intestinal contents, tissues, or swab in anaerobic transport media
Species
Sampling Instructions
Collect sample aseptically. Do not add formalin or other liquids.
Collection Container
Sterile, sealed, leak-proof container
Packing Instructions
Label samples and ship with cold packs.
Special Instructions
Swabs must be in anaerobic transport media.
Transport media can be purchased from WVDL.
WVDL Microbiology Media Order Form can be found on our website. [include link]
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Barron
Monday, Tuesday, Wednesday, Thursday, Friday
Results completed in 5-12 days.
WVDL - Madison
Monday, Tuesday, Wednesday, Thursday, Friday
Results completed in 5-12 days.
Clostridium perfringens spp Real Time PCR
Detection of Clostridium perfringens spp by real-time PCR
30848 Cattle, Goats, Sheep, Gazelle, Antelope, other Bovids, and Horses CPERFSPPCR colitis standard neonate equine enteric
Cattle, Goats, Sheep, Gazelle, Antelope, other Bovids, and Horses
Sampling Instructions
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Companion Gram Negative Susceptibility testing using MIC
Testing on bacterial isolates using automated Sensititre instrumentation. Panels available for bovine / porcine, companion / equine, mastitis and avian isolates. Campylobacter and anaerobic panels available upon request. See Susceptibility-Mastitis for specific information on mastitis susceptbility panel. Call lab for specific information.
11132 Cats, Dogs, Rabbits, Rodents, and Mustelids SUSCEPTMIC KIRBY BAUER MINIMUM INHIBITORY CONCENTRATION (MIC) SENSITIVITY SUSCEPTIBILITY BY SENSITITRE Streptococcus species cat dog feline canine k9
Innoculate single well isolated colony onto sterile media that supports target's growth.
Non selective media is reccomended (BAP, Chocolate, TSA)
Collection Container
Appropriate culture media that supports bacterial growth (culture plate/slant)
Packing Instructions
Label samples and ship with cold packs.
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Tuesday, Wednesday, Thursday
Companion Gram Positive Susceptibility testing using MIC
Testing on Streptococcus species isolated from milk samlpes using automated Sensititre instrumentation. Panel includes ampicillin, penicillin, erythromycin, oxacillin, pirlimycin, penicillin/novobiocin, tetracycline, cephalothin, ceftiofur and sulfadimethoxine
11145 Cats, Dogs, Rabbits, Rodents, and Mustelids SUSCEPTMIC KIRBY BAUER MINIMUM INHIBITORY CONCENTRATION (MIC) SENSITIVITY SUSCEPTIBILITY BY SENSITITRE cat dog feline canine k9
Innoculate single well isolated colony onto sterile media that supports target's growth.
Non selective media is reccomended (BAP, Chocolate, TSA)
Collection Container
Appropriate culture media that supports bacterial growth (culture plate/slant)
Packing Instructions
Label samples and ship with cold packs.
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Tuesday, Wednesday, Thursday
Count of Bacteria from Extended Semen
Aerobic count of 0.1mL of semen.
Culture plates incubated for at least 72 hours.
Maximum of two bacterial identifications made on suspect colonies.
More identications may be requested with additional fees.
The Q Fever CF Complement Fixation test is utilized for detecting antibodies to Coxiella burnetii in bovine serum. Bovine serum only. All non-bovine samples are referred to NVSL.
1-3 mL serum or 3-5 mLs clotted blood. If requesting 3 or more serological assays, please submit 10 mLs serum to accommodate all tests requested.
Species
Cattle
Sampling Instructions
Collection Container
Collect serum from clotted blood in a non-additive tube (e.g. plain red top or serum separator). Excessively hemolyzed samples are not acceptable for this test.
Packing Instructions
Refrigerate, ship with cold packs overnight
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
-
Coxiella burnetii (Q Fever) ELISA
The Q Fever ELISA is utilized to detect antibodies to Coxiella burnetii in bovine serum. This kit manufacturer has validated this test for bovine serum/milk, caprine serum/milk and ovine serum. Bovine milk and non-bovine serum/milk can be tested but is not covered under the WVDL's standard operating procedures. Non-bovine specimens will be reported with a disclaimer. This QF ELISA kit is not USDA licensed, but is validated by the WVDL for use. The Q-fever complement fixation (CF) assay is still available at the WVDL.
Collect serum from clotted blood in a non-additive tube (e.g. plain red top or serum separator). Excessively hemolyzed samples are not acceptable for this test.
Packing Instructions
Refrigerate, ship with cold packs overnight
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
-
Coxiella burnetii Real Time PCR
Detection of Coxiella burnetii by real-time PCR
30861 Cattle, Goats, Sheep, Gazelle, Antelope, other Bovids, and Camelids including Alpacas and Llamas QFEVERPCR Australian Q fever abattoir fever Balkan influenza pneumorickettsiosis nine-mile fever QF
Cattle, Goats, Sheep, Gazelle, Antelope, other Bovids, and Camelids including Alpacas and Llamas
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Package placenta separtely from other samples.
Label all samples.
Ship overnight on cold packs in an insulated container.
Special Instructions
Samples being test for Coxiella are processed in a BSL3 laboratory, this may affect test turnaround times.
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hours of test set up.
Coxiella burnetii Real Time PCR (Semen/Embryo)
Detection of Coxiella burnetii by real-time PCR in semen and embryo wash fluids.
30874 Cattle QFEVERPCR QF Q-fever abortion export regulatory New Zealand
Semen (200uL Extended semen: 4 x 250cc or 2 x 500cc straws)
1 ml embryo wash fluid
Species
Cattle
Sampling Instructions
Semen samples should be sent frozen in liquid nitrogen. Embyro wash fluid may be kept refrigerated until shipping.
Collection Container
Leak-proof tubes or straws.
Packing Instructions
Please utilize a semen tank map and place semen into numerically identified holders that correspond to the paperwork. Bundle semen straws for PCR separately from semen straws for virus isolation or culture testing. Using separate semen tanks for each section is ideal. Embyro wash fluids should be sent in leak-proof containers chilled on ice packs.
Special Instructions
This test is specific for semen and/or embryo samples. We cannot pool semen straws from different collection dates.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Tuesday
Results available within 24 hours of test set up.
Cryptosporidium parvum Real Time PCR
Detection of Cryptosporidium parvum by real-time PCR
30887 Cattle, Goats, Sheep, Gazelle, Antelope, other Bovids, Deer, Horses, and Camelids including Llamas and Alpaca CRYPTOPCR protozoa parasite enteric water
Cattle, Goats, Sheep, Gazelle, Antelope, other Bovids, Deer, Horses, and Camelids including Llamas and Alpaca
Sampling Instructions
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Wednesday, Friday
Results available within 24 hours of test set up.
Cryptosporidium Stain
Direct sample staining for the detection of Cyrptospordia species
10300 CRYPTSTAIN cryptosporidia crypto stain acid fast af stain
Submit obex or retropharyngeal lymph node or tonsil in a leak proof jar containing 10% neutral buffered formalin.
Species
Cervids
Sampling Instructions
Formalin fixed tissue
Collection Container
Leakproof jar containing 10% neutral buffered formalin at a ratio of 10 times the volume of tissue.
Packing Instructions
Each container must be labeled with the official identification that is human readable (minimum) and a barcode (best practice) on the side of the container. Additionally, the top of the jar must be labeled with the position number of the associated submission.
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Tuesday, Wednesday, Thursday, Friday
Within 10 business days
CWD IHC - 2 Animals Per Slide
Detection of Chronic Wasting Disease in Cervids with IHC
Submit obex or retropharyngeal lymph node or tonsil in a leak proof jar containing 10% neutral buffered formalin.
Species
Cervids
Sampling Instructions
Formalin fixed tissue
Collection Container
Leak-proof jar containing 10% neutral buffered formalin at a ratio of 10 times the volume of tissue.
Packing Instructions
Each container must be labeled with the official identification that is human readable (minimum) and a barcode (best practice) on the side of the container. Additionally, the top of the jar must be labeled with the position number of the associated submission.
Special Instructions
This price is per animal when submitted in multiples of 2. If an odd number of animals are submitted then one will be charged the 1 per slide price (70020)
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Tuesday, Wednesday, Thursday, Friday
Within 10 business days
CWD IHC - Biopsy
Detection of Chronic Wasting Disease in Cervids Antemortem samples with IHC
Submit rectoanal mucosa-associated lymphoid tissue (RAMALT) or tonsil biopsy in a leak proof jar containing 10% neutral buffered formalin.
Species
Cervids
Sampling Instructions
Formalin fixed tissue
Collection Container
Leak-proof jar containing 10% neutral buffered formalin at a ratio of 10 times the volume of tissue.
Packing Instructions
Each container must be labeled with the official identification that is human readable (minimum) and a barcode (best practice) on the side of the container. Additionally, the top of the jar must be labeled with the position number of the associated submission.
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Tuesday, Wednesday, Thursday, Friday
Within 10 business days
CWD IHC - Obex and Lymph Node
Detection of Chronic Wasting Disease in Cervids with IHC
Submit obex and half of each retropharyngeal lymph node in a leak proof jar containing 10% neutral buffered formalin. Package the eartags attached to a quarter sized piece of ear, half of each lymph node and two pieces of brain stem (caudal and cranial to the obex) in a ziploc bag and freeze. The frozen samples can either be sent with the fixed samples to WVDL or retained at your clinic.
Species
Cervids
Sampling Instructions
Formalin fixed tissue
Collection Container
Leak-proof jar containing 10% neutral buffered formalin at a ratio of 10 times the volume of tissue.
Packing Instructions
Each container must be labeled with the official identification that is human readable (minimum) and a barcode (best practice) on the side of the container. Additionally, the top of the jar must be labeled with the position number of the associated submission.
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Tuesday, Wednesday, Thursday, Friday
10 Days
Deer Head CWD ELISA - Wild
Sample harvest and detection of Chronic Wasting Disease in wild Cervids with ELISA
Remove the head from the carcass leaving about 4 inches of the neck attached. Label each outer bag (prior to placing the head inside) with the following information: Date of collection & Sample ID. Freeze the head until ready to ship.
Species
Cervids
Sampling Instructions
Frozen / Cold head with 4 inches of neck
Collection Container
Double-bag the head in plastic bags and tie off or zip tie each bag to seal.
Packing Instructions
On day of shipment, place frozen lymph nodes or head in a hard-sided shipping container or cooler. Include a frozen ice (gel) pack to keep the sample cool. Please do not use regular ice as it will melt and cause damage to the included materials. Pack the container with paper towel or newspaper to keep contents from shifting.
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Tuesday, Wednesday, Thursday, Friday
5 Days
Deer Head CWD IHC - Captive
Sample harvest and detection of Chronic Wasting Disease in captive Cervids with IHC
Remove the head from the carcass leaving about 4 inches of the neck attached. Label each outer bag (prior to placing the head inside) with the following information: Date of collection & Sample ID. Freeze the head until ready to ship.
Species
Cervids
Sampling Instructions
Frozen / Cold head with 4 inches of neck
Collection Container
Double-bag the head in plastic bags and tie off or zip tie each bag to seal.
Packing Instructions
On day of shipment, place frozen lymph nodes or head in a hard-sided shipping container or cooler. Include a frozen ice (gel) pack to keep the sample cool. Please do not use regular ice as it will melt and cause damage to the included materials. Pack the container with paper towel or newspaper to keep contents from shifting.
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
E. coli Virulence Factor K99/Intimin/F41/Sta/Stx1/Stx2 PCR Panel
Detection of E. coli Virulence Factor K99/Intimin/F41/Sta/Stx1/Stx2 Panel by real-time PCR
30991 Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids
Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids
Sampling Instructions
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Eastern equine encephalitis virus/West Nile virus PCR Panel
Detection of Eastern equine encephalitis virus (EEE) and West Nile virus (WNV) by real-time PCR
31017 Birds and Equines EEEWNVPNL triple E sleeping sickness vector neurologic WNV horses
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Samples being tested for EEE/WNV are processed in a BSL3 laboratory, this may affect test turnaround times.
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Ectoparasite Exam
Macro and micro inspcection of samples for ectopic parasites including lice, fleas, mites and ticks
10365 PARA-ECTO external parasite lice mange paraecto ticks fleas
Frozen Semen. Minimum of 4 straws per collection; 0.5ml non-extended semen
Species
Cattle
Sampling Instructions
Collection date and clearly labeled EDTA blood tubes or semen straws in liquid nitrogen.
Collection Container
Packing Instructions
Special Instructions
A shipping fee will be applied to the accession. Testing is performed at NVSL and takes an estimated 22 business days to complete. Please see their website for updated testing days of the week and turn around time.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Wednesday, Thursday, Friday
22 Business days
WVDL - Barron
Wednesday, Thursday, Friday
22 Business Days
Electron Microscopy Negative Stain
Evaluation of samples for virus particles using transmission electron microscopy by negative stain.
40563 EMNEGSTAIN EM Electron Microscopy Negative Stain Transmission electron microscopy TEM
Please contact the Virology section prior to submission.
Species
Sampling Instructions
Please contact the Virology section prior to submission.
Collection Container
Please contact the Virology section prior to submission.
Packing Instructions
Please contact the Virology section prior to submission.
Special Instructions
Please contact the Virology section prior to submission.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
-
As needed
Epizootic hemorrhagic disease virus by Agar Gel Immunodiffusion (AGID)
Agar gel immunodiffusion is utilizied for the detection of EHD antibodies and is often required for import or export of cattle or bovine. EHD and BTV have known cross-reactivity on AGIDs. Susceptible species: sheep, cattle, ruminant animals. (Bovine, Bovine other, Caprine, Ovine)
20353 Cattle Agar Gel Immunodiffusion Assay EHD Epizootic Hemorrhagic Disease AGID
1-3 mL serum or 3-5 mLs clotted blood. If requesting 3 or more serological assays, please submit 10 mLs serum to accommodate all tests requested.
Species
Cattle
Sampling Instructions
Collection Container
Collect serum from clotted blood in a non-additive tube (e.g. plain red top or serum separator). Excessively hemolyzed samples are not acceptable for this test.
The EHD cELISA is utilized for the detection of EHD antibodies in bovine sera. This kit manufacturer has validated this test for serum/plasma from bovine, ovine, caprine and deer species. Non-bovine species and plasma can be tested but is not covered under the WVDL's standard operating procedures. Non-bovine specimens will be reported with a disclaimer.
20366 Cattle. Goats and Sheep can be ran with disclaimer. EHD Epizootic Hemorrhagic Disease
1-3 mL serum or 3-5 mLs clotted blood. If requesting 3 or more serological assays, please submit 10 mLs serum to accommodate all tests requested.
Species
Cattle. Goats and Sheep can be ran with disclaimer.
Sampling Instructions
Collection Container
Collect serum from clotted blood in a non-additive tube (e.g. plain red top or serum separator). Excessively hemolyzed samples are not acceptable for this test.
Packing Instructions
Refrigerate, ship with cold packs overnight
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
-
Epizootic hemorrhagic disease virus Real Time PCR (Semen)
Detection of Epizootic hemorrhagic disease virus by real-time PCR in semen
Semen (200uL Extended semen: 4 x 250cc or 2 x 500cc straws)
Species
Cattle
Sampling Instructions
Semen samples should be sent frozen in liquid nitrogen.
Collection Container
Leak-proof tubes or straws.
Packing Instructions
Please utilize a semen tank map and place semen into numerically identified holders that correspond to the paperwork. Bundle semen straws for PCR separately from semen straws for virus isolation or culture testing. Using separate semen tanks for each section is ideal.
Special Instructions
This test is specific for semen samples. We cannot pool semen straws from different collection dates.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Tuesday
Results available within 24 hours of test set up.
Epizootic hemorrhagic disease virus Type 1 & Type 2 Serum Neutralization
Detection of antibodies to epizootic hemorrhagic disease (EHD) virus Type 1 & Type 2 by serum Neutralization.
40134 Cattle EPIZOOTIC HEMORRHAGIC DISEASE EHD SN T1 EHD SN T2 EHD1&2SN EHD
Collect serum from clotted blood in a non-additive tube. Avoid hemolysis.
Collection Container
Serum samples should be submitted in serum separator tubes, uncoated/plain tubes may be appropriate for spun serum.
Packing Instructions
Label all samples with unique identifiers. Ship overnight on cold packs.
Special Instructions
Submit paired (acute & convalescent) serum samples together. Convalescent samples should be collected 10 to 14 days after the acute animal has been sampled. Separate acute serum from clot and store frozen until the convalescent sample has been collected. Comparison of sera from 2 points in time requires testing on the same day.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Thursday
Results available Monday
Epizootic hemorrhagic disease virus Type 1 & Type 2 Serum Neutralization (titered to endpoint)
Detection of antibodies to epizootic hemorrhagic disease (EHD) virus Type 1 & Type 2 by serum Neutralization. Serum is titered to endpoint.
40147 Cattle EPIZOOTIC HEMORRHAGIC DISEASE EHD SN T1 EHD SN T2 EHD
Collect serum from clotted blood in a non-additive tube. Avoid hemolysis.
Collection Container
Serum samples should be submitted in serum separator tubes, uncoated/plain tubes may be appropriate for spun serum.
Packing Instructions
Label all samples with unique identifiers. Ship overnight on cold packs.
Special Instructions
Submit paired (acute & convalescent) serum samples together. Convalescent samples should be collected 10 to 14 days after the acute animal has been sampled. Separate acute serum from clot and store frozen until the convalescent sample has been collected. Comparison of sera from 2 points in time requires testing on the same day.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Thursday
Results available Monday
Equine Abortion Culture Panel
Culture for aerobic bacteria known to cause abortions including Salmonella and fungal culture
Mycoplasmopsis and Ureaplamsa not included.
Collect sample aseptically. Do not add formalin or other liquids.
Collection Container
Sterile, sealed, leak-proof container
Packing Instructions
Label samples and ship with cold packs.
Special Instructions
Swabs must be in transport media
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Barron
Monday, Tuesday, Wednesday, Thursday, Friday
Results completed in 5-12 days.
WVDL - Madison
Monday, Tuesday, Wednesday, Thursday, Friday
Results completed in 5-12 days.
Equine Abortion PCR Panel
Detection of pathogens commonly attributed to abortion in equines; includes Equine herpesvirus Type 1 (EHV-1), Equine viral arteritis virus (EAV), Leptospira spp by real-time PCR.
31056 Equines, including Horses, Donkeys, Mules, and Zebras ABORPNLEQ EAV EHV-1 LEPTO
Equines, including Horses, Donkeys, Mules, and Zebras
Sampling Instructions
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Package placenta in a separately than other samples.
Label all samples.
Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Equine arteritis virus Real Time PCR
Detection of Equine arteritis virus by real-time PCR
31069 Equines, including Horses, Donkeys, Mules, and Zebras EVAPCR respiratory reproductive abortion
Equines, including Horses, Donkeys, Mules, and Zebras
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Package placenta in a separately than other samples.
Label all samples.
Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Equine herpesvirus Type 1 Real Time PCR
Detection of Equine herpesvirus Type 1 by real-time PCR
31082 Equines and Camelids including Alpacas and Llamas EHV-1PCR abortion respiratory neurologic encephalitis Horses
Nasal swabs in viral transport media or 1-3ml sterile saline is preferred. It is recommended to submit both EDTA and nasal swabs from affected horses. If media not available, add 1-2 drops of sterile saline to the swab. Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing. Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Package placenta in a separately than other samples.
Label all samples.
Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Equine herpesvirus Type 4 Real Time PCR
Detection of Equine herpesvirus Type 4 by real-time PCR
31095 Equines, including Horses, Donkeys, Mules, and Zebras EHV-4PCR respiratory rhinopneumonitis
Equines, including Horses, Donkeys, Mules, and Zebras
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Package placenta in a separately than other samples.
Label all samples.
Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Equine infectious anemia virus ELISA
EIA Antibody ELISA
20379 Equines, including Horses, Donkeys, Mules, and Zebras COGGINS TEST EQUINE INFECTIOUS ANEMIA SWAMP FEVER EIA
Serum, 1 ml; clearly identify the animal ID on the serum sample, which must match the submission form. Hemolyzed samples will not be tested.
Species
Equines, including Horses, Donkeys, Mules, and Zebras
Sampling Instructions
NO ACCESSION FEE. Official EIA submission form required with all submissions: VS-10, Global Vet Link or VSPS accepted. Submitting veterinarian must have valid NAN. Submissions forms not fully completed maybe be charged an additional fee and testing may be delayed. More information found on EIA web page: https://www.wvdl.wisc.edu/documents/equine-infections-anemia-eia-services-and-submissions/
Collection Container
Packing Instructions
Refrigerate; ship with cold packs
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Barron
-
Equine Neurologic PCR Panel
Detection of pathogens commonly attributed to neurologic symptoms in equines; includes Equine herpesvirus Type 1 (EHV-1), Eastern equine encephalitis virus (EEE) and West Nile virus (WNV) by real-time PCR
31121 Equines, including Horses, Donkeys, Mules, and Zebras NEUROPNLEQ encephalitis EHV-1 EEE WNV
Equines, including Horses, Donkeys, Mules, and Zebras
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Collect sample aseptically. Do not add formalin or other liquids.
Collection Container
Sterile, sealed, leak-proof container
Packing Instructions
Label samples and ship with cold packs.
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Barron
Monday, Tuesday, Wednesday, Thursday, Friday
Results completed in 5-12 days.
WVDL - Madison
Monday, Tuesday, Wednesday, Thursday, Friday
Results completed in 5-12 days.
Equine Respiratory PCR Panel
Detection of pathogens commonly attributed to respiratory disease in equines; includes Equine herpesvirus Type 1 (EHV-1), Equine herpesvirus Type 4 (EHV-4), Influenza A, Streptococcus equi equi, Rhodococcus equi and Equine viral artertis (EAV) by real-time PCR
31147 Equines, including Horses, Donkeys, Mules, and Zebras RESPNLEQ EHV-1 EHV-4 EAV FluA S. equi R. equi
Equines, including Horses, Donkeys, Mules, and Zebras
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Equine Susceptibility testing using MIC
Testing on bacterial isolates using automated Sensititre instrumentation. Panels available for bovine / porcine, companion / equine, mastitis and avian isolates. Campylobacter and anaerobic panels available upon request. See Susceptibility-Mastitis for specific information on mastitis susceptbility panel. Call lab for specific information.
11184 Equines including Horses, Mules, Donkeys, and Zebra SUSCEPTMIC KIRBY BAUER MINIMUM INHIBITORY CONCENTRATION (MIC) SENSITIVITY SUSCEPTIBILITY BY SENSITITRE Staphylococcus species horse equine foal zebra
Collect sample aseptically. Do not add formalin or other liquids.
Collection Container
Sterile, sealed, leak-proof container.
Do not submit in gloves or OB sleeves.
Packing Instructions
Label samples and ship with cold packs. DO NOT FREEZE
Special Instructions
Avian pooled/composite feces for fecal flotation will be accepted if pre-pooled.
Pooled samples are not validated for this SOP and will have a disclaimer.
Feces submitted in transportation media such as ParaPak can be run but cannot be semiquantified and will have a disclaimer.
1-3 ml swabs: conjunctival, nasal, oral/pharyngeal, tracheal
1g feces
1g tissues: brain, intestine, lung
Species
Cats
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Feline Enteric PCR Panel
Detection of pathogens commonly attributed to enteric disease in felines; includes Feline coronavirus (FCoV) and Feline panleukopenia virus (FPL) by real-time PCR
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
1-3 ml swabs: conjunctival, nasal, oral/pharyngeal, tracheal
1g tissues: lung
Species
Cats
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Feline panleukopenia virus Real Time PCR
Detection of Feline panleukopenia virus by real-time PCR
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Feline Respiratory PCR Panel
Detection of pathogens commonly attributed to respiratory disease in felines: includes Feline calcivirus (FCV), Feline coronavirus (FCoV), Feline herpesvirus (FHV), Influenza A (Flu-A), Chlamydia spp, and Mycoplasma felis by real-time PCR
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Foot and mouth disease virus Real Time PCR
Detection of foot and mouth disease (FMD) virus by real time PCR.
Vesicular tissue, oral or lesion swab in tris-buffered tryptose broth (TBTB). Swabs should be submitted in a minimum of 3 ml TBTB.
Species
Cattle, Sheep, Goats, Pigs, Camelids, Cervids
Sampling Instructions
Swabs should be submitted in TBTB. Swabs must be removed from media. Swirl each swab in the liquid, press the tip of the swab on the side of the tube to release remaining liquid.
Collection Container
Swabs or tissues should be submitted in a labeled, sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs to arrive within 48 hours of collection.
Special Instructions
NAHLN assay. Test is performed for Foreign Animal Disease (FAD) investigations. Contact AVIC and DATCP prior to WVDL submission. Non-Negative results are confirmed at NVSL-FADDL.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
-
As needed. TAT will be based on FADI priority level.
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Fungal Culture
Culture for fungi, yeast, and includes special media or extended incubation for growth of dermatophytes and sytemic fungi such as Blastomyces and Histoplasma.
10469 FUNGALCULT fungus fungal culture fungal screen dermatophytes microsporum canis ring worm dermatophytosis trichophyton verrucosum trichophyton species microsporum species
Innoculate single well isolated colony/growth onto sterile media that supports target organism.
IMA or SAB-DEX media recommended
Collection Container
Appropriate culture media that supports bacterial growth (culture plate/slant)
Packing Instructions
Label samples and ship with cold packs.
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Barron
Monday, Tuesday, Wednesday, Thursday, Friday
Results completed in 5-12 days.
WVDL - Madison
Monday, Tuesday, Wednesday, Thursday, Friday
Results completed in 5-12 days.
Fungal Screen Culture
Culture for rapidly growing fungi and yeast.
Does not include special media or extended incubation for growth of dermatophytes and sytemic fungi such as Blastomyces and Histoplasma.
10495 FUNALSCR Aspergillus species Mucoralis group Mucoralis species Penicillium species Fusarium species
Collect sample aseptically. Do not add formalin or other liquids.
Collection Container
Sterile, sealed, leak-proof container
Packing Instructions
Label samples and ship with cold packs.
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Barron
Monday, Tuesday, Wednesday, Thursday, Friday
Results completed in 5-12 days.
WVDL - Madison
Monday, Tuesday, Wednesday, Thursday, Friday
Results completed in 5-12 days.
General Abortion Panel
Culture for aerobic and microaerphilic bacteria known to cause abortions including Listeria, Campylobacter, Salmonella
Mycoplasmopsis and Ureaplamsa not included.
(Refer to specific tests for Bovine Group, Equine, Porcine, Canine and Feline)
2-3 ml fluids: bronchoalveloar, transtracheal wash, pleural fluid
1g tissues: brain, lung
Species
Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Wednesday, Friday
Results available within 24 hours of test set up.
IAV/APMV-1 Real Time PCR Panel
Detection of influenza A (IAV) virus and avian paramyxovirus type 1 (APMV-1) by multiplexing real time PCR.
32408 Birds NAHLN, AIENDPNL, AI MATRIX, AI PCR, INFLUENZA, AVIAN, HPAI, AVIAN FLU, BIRD FLU, INFLUENZA A VIRUS, APMV, AVIAN, PARAMYXOVIRUS
Oropharyngeal swab (may pool up to 5), tracheal swab (may pool up to 5), cloacal swab (may pool up to 5), cloacal & oropharyngeal swab pool from 1 bird, tissue samples pooled from 1 bird.Pooled swabs should be submitted in 3ml (5 swabs) or in 5.5 ml (up to 11 swabs) Viral Transport Media (VTM) or Brain heart infusion broth (BHI).
Species
Birds
Sampling Instructions
Swabs should be submitted in viral transport media or BHI both. Swabs must be removed from media. Swirl each swab in the liquid, press the tip of the swab on the side of the tube to release remaining liquid.
Collection Container
BHI broth, viral transport media or swabs should be submitted in a labeled, sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs to arrive within 48 hours of collection.
Special Instructions
NAHLN assay. Subtyping may be performed if the AI Matrix result is non-negative. Non-negative results are confirmed at NVSL.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Tuesday, Wednesday, Thursday, Friday
As needed. Testing will be completed M-F within 48 hrs of receipt.
Choanal, cloacal, nasal, oral, pharyngeal, or tracheal swab in 1-3 ml media (VTM, BHI, PBS)
1 g Lung
Species
Birds
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Infectious laryngotracheitis virus Real Time PCR
Detection of Infectious laryngotracheitis virus by real-time PCR
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Influenza A virus (Bovine) antibody ELISA
Detection of antibodies to Influenza A virus (IAV) in cattle by ELISA.
40576 Cattle ELISA INFLUENZA bovine ai NAHLN, IAV, INFLUENZA, BOVINE, HPAI, INFLUENZA A VIRUS, CATTLE, MILK, ELISA
Collect serum from clotted blood in a non-additive tube. Avoid hemolysis.
Collection Container
Serum samples should be submitted in serum separator tubes, uncoated/plain tubes may be appropriate for spun serum. Milk should be sent in a leak proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs.
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available same day, unless confirmation is needed.
Influenza A virus (Swine) Real Time PCR
Detection of influenza A virus in swine (IAV-S) by real time PCR.
Swabs should be submitted in 3 ml Viral Transport Media (VTM) or Brain heart infusion broth (BHI). Swabs must be removed from media. Swirl each swab in the liquid, press the tip of the swab on the side of the tube to release remaining liquid.
Collection Container
BHI broth, viral transport media or swabs should be submitted in a labeled, sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs to arrive within 48 hours of collection.
Special Instructions
NAHLN assay. Non-Negative results are confirmed at NVSL.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
-
Influenza A virus by Agar Gel Immunodiffusion (AGID)
Avian Influenza by AGID
20067 Chicken, Turkey, Pheasant, Quail, and other Galliformes
Oropharyngeal swab (may pool up to 5), tracheal swab (may pool up to 5), cloacal swab (may pool up to 5), cloacal & oropharyngeal swab pool from 1 bird, tissue samples pooled from 1 bird. Pooled swabs should be submitted in 3ml (5 swabs) or in 5.5 ml (up to 11 swabs) Viral Transport Media (VTM) or Brain heart infusion broth (BHI).
Species
Birds, Cattle
Sampling Instructions
Swabs should be submitted in viral transport media or BHI both. Swabs must be removed from media. Swirl each swab in the liquid, press the tip of the swab on the side of the tube to release remaining liquid.
Collection Container
BHI broth, viral transport media or swabs should be submitted in a labeled, sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs to arrive within 48 hours of collection.
Special Instructions
NAHLN assay. Subtyping may be performed if the AI Matrix result is non-negative. Non-negative results are confirmed at NVSL.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Tuesday, Wednesday, Thursday, Friday
As needed. Testing will be completed within 48 hrs of receipt.
Influenza A virus Real Time PCR
Detection of Influenza A virus by real-time PCR
31381 All Mammals including Dogs, Cats, Horses, Ferrets, and Mink FLU-A-PCR respiratory
All Mammals including Dogs, Cats, Horses, Ferrets, and Mink
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Influenza D virus Real Time PCR
Detection of Influenza D virus by real-time PCR
31407 Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids FLUDPCR BRD IDV respiratory
Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Wednesday, Friday
Results available within 24 hours of test set up.
Intact Parasite Identification
10729 ectoparasite endoparasite parasite ID live parasite Ticks Lice Mites
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Leptospira Antibody Microagglutination Test (LeptoMAT) (5 Serovars) reported at a minimum of 1:100
The 5 Serovar Leptospirosis test for bull stud clients. Serovars included are Canicola, Grippotyphosa, Hardjo, Icterohaemorrhagiae and Pomona. Serologic diagnosis requires detection of a significant rise in antibody titer in paired serum samples 10-14 days apart.
Serum;1 mL minimum. If multiple tests are required, then a 1-2 day delay should be expected. Sending in additional sera samples will remove that delay.
Species
Mammals
Sampling Instructions
Collection Container
Collect serum from clotted blood in a non-additive tube (e.g. plain red top or serum separator). Excessively hemolyzed samples are not acceptable for this test.
Packing Instructions
Refrigerate, ship with cold packs overnight
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
-
Leptospira Antibody Microagglutination Test (LeptoMAT) (5 Serovars) reported at a minimum of 1:400
The 5 Serovar Leptospirosis test for bull stud clients. Serovars included are Canicola, Grippotyphosa, Hardjo, Icterohaemorrhagiae and Pomona. Serologic diagnosis requires detection of a significant rise in antibody titer in paired serum samples 10-14 days apart.
Serum;1 mL minimum. If multiple tests are required, then a 1-2 day delay should be expected. Sending in additional sera samples will remove that delay.
Species
Mammals
Sampling Instructions
Collection Container
Collect serum from clotted blood in a non-additive tube (e.g. plain red top or serum separator). Excessively hemolyzed samples are not acceptable for this test.
Packing Instructions
Refrigerate, ship with cold packs overnight
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
-
Leptospira Antibody Microagglutination Test (LeptoMAT) (6 Serovars) reported at a minimum of 1:100
The 6 Serovar Leptospirosis Test Serovars included are Canicola, Grippotyphosa, Hardjo, Icterohaemorrhagiae, Pomona and Bratislava. Serologic diagnosis requires detection of a significant rise in antibody titer in paired serum samples 10-14 days apart.
Serum;1 mL minimum. If multiple tests are required, then a 1-2 day delay should be expected. Sending in additional sera samples will remove that delay.
Species
Mammals
Sampling Instructions
Collection Container
Collect serum from clotted blood in a non-additive tube (e.g. plain red top or serum separator). Excessively hemolyzed samples are not acceptable for this test.
Packing Instructions
Refrigerate, ship with cold packs overnight
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
-
Leptospira Antibody Microagglutination Test (LeptoMAT) (6 Serovars) reported at a minimum of 1:400
The 6 Serovar Leptospirosis Test Serovars included are Canicola, Grippotyphosa, Hardjo, Icterohaemorrhagiae, Pomona and Bratislava. Serologic diagnosis requires detection of a significant rise in antibody titer in paired serum samples 10-14 days apart.
Serum;1 mL minimum. If multiple tests are required, then a 1-2 day delay should be expected. Sending in additional sera samples will remove that delay.
Species
Mammals
Sampling Instructions
Collection Container
Collect serum from clotted blood in a non-additive tube (e.g. plain red top or serum separator). Excessively hemolyzed samples are not acceptable for this test.
Packing Instructions
Refrigerate, ship with cold packs overnight
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
-
Leptospira automnalis Microagglutination Test (LeptoMAT)
This test is primarily used to meet export requirements; not used as a routine diagnostic test.
Collect serum from clotted blood in a non-additive tube (e.g. plain red top or serum separator). Excessively hemolyzed samples are not acceptable for this test.
Packing Instructions
Refrigerate, ship with cold packs overnight
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
-
Leptospira OIE Microagglutination Test (LeptoMAT) Confirmation Assay
This test is primarily used to meet export requirements; not used as a routine diagnostic test.
Serum;1 mL minimum. If multiple tests are required, then a 1-2 day delay should be expected. Sending in additional sera samples will remove that delay.
Species
Mammals
Sampling Instructions
Collection Container
Collect serum from clotted blood in a non-additive tube (e.g. plain red top or serum separator). Excessively hemolyzed samples are not acceptable for this test.
Packing Instructions
Refrigerate, ship with cold packs overnight
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
-
Leptospira sejroe Microagglutination Test (LeptoMAT)
This test is primarily used to meet export requirements; not used as a routine diagnostic test.
Serum;1 mL minimum. If multiple tests are required, then a 1-2 day delay should be expected. Sending in additional sera samples will remove that delay.
Species
Mammals
Sampling Instructions
Collection Container
Collect serum from clotted blood in a non-additive tube (e.g. plain red top or serum separator). Excessively hemolyzed samples are not acceptable for this test.
Cattle, Horses, Sheep, Goats, Cats, Dogs, Camelids including Llamas and Alpacas
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Package placenta in a separately than other samples.
Label all samples.
Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Wednesday, Friday
Results available within 24 hours of test set up.
Leptospira spp. Real Time PCR (Semen/Embryo)
Detection of Leptospira spp. By real-time PCR in semen and embryo wash fluid
Semen (200uL Extended semen: 4 x 250cc or 2 x 500cc straws)
1 ml embryo wash fluid
Species
Cattle
Sampling Instructions
Semen samples should be sent frozen in liquid nitrogen. Embyro wash fluid may be kept refrigerated until shipping.
Collection Container
Leak-proof tubes or straws.
Packing Instructions
Please utilize a semen tank map and place semen into numerically identified holders that correspond to the paperwork. Bundle semen straws for PCR separately from semen straws for virus isolation or culture testing. Using separate semen tanks for each section is ideal. Embyro wash fluids should be sent in leak-proof containers chilled on ice packs.
Special Instructions
This test is specific for semen and/or embryo samples. We cannot pool semen straws from different collection dates.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Tuesday
Results available within 24 hours of test set up.
Listeria Culture
Culture for Listeria species
10573 LISTSCREEN circling disease listeriosis L. monocytogenes L. mono. L mono L. mono List mono List monocytogenes Listeria monocytogenes Listeria species Listeria sp. List. sp. List. Mono abortion
Cats, Cattle, Goats, Sheep, Gazelle, Antelope, other Bovids, and Sugar Gliders
Sampling Instructions
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Package placenta in a separately than other samples.
Label all samples.
Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
2-3 ml fluids: bronchoalveloar fluid, transtracheal wash
1-32 ml swabs: nasal, oral/pharyngeal, tracheal
1g tissues: lung
Species
Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Wednesday, Friday
Results available within 24 hours of test set up.
Mastitis Bulk Tank Culture Panel
Culture for organims known to cause mastitis from bulk tank milk samples.
Includes culture for Prototheca and Mycoplasmopsis
10521 MASTBT bulk tank milk bulk tank culture mastitis milk protheca mycoplasma mycplasmopsis mycoplasmoides
Prior to sample collection, agitate the bulk tank milk for 10 minutes. Take samples from the top of the tank with a sterile syringe and needle. Remove needle and transfer contents (15-20 cc's) of milk into a sterile plastic vial. Freeze immediately.For optimal cuture results, 3-5 consecutive samples are recommended. (Will be pooled at laboratory). Use Milk Culture Submission form.
Collection Container
Packing Instructions
Label samples and ship with cold packs.
Special Instructions
Samples arriving on Thursday and Friday are frozen and cultured on Monday of the following week.
Note: Internal data demonstrates a significant loss of recovery of Prototheca when the sample is frozen. If the sample was frozen, the use of those results for any purpose is at the discretion of the submitter.
Collect sample aseptically. Do not add formalin or other liquids.
Collection Container
Sterile, sealed, leak-proof container
Packing Instructions
Label samples and ship with cold packs.
Special Instructions
Does not include Mycoplasma. [LINK]
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Barron
Monday, Tuesday, Wednesday, Thursday, Friday
Results completed in 5-12 days.
WVDL - Madison
Monday, Tuesday, Wednesday, Thursday, Friday
Results completed in 5-12 days.
Mastitis Susceptibility testing using MIC
Testing on bacterial isolates using automated Sensititre instrumentation. Panels available for bovine / porcine, companion / equine, mastitis and avian isolates. Campylobacter and anaerobic panels available upon request. See Susceptibility-Mastitis for specific information on mastitis susceptbility panel. Call lab for specific information.
10898 Bovidae and Camelidae Family including Cattle, Sheep, Goats, Llamas, Alpaca, Gazelle, and Antelope SUSCEPTMIC KIRBY BAUER MINIMUM INHIBITORY CONCENTRATION (MIC) SENSITIVITY SUSCEPTIBILITY BY SENSITITRE cattle goat milk mastitis
Bovidae and Camelidae Family including Cattle, Sheep, Goats, Llamas, Alpaca, Gazelle, and Antelope
Sampling Instructions
Innoculate single well isolated colony onto sterile media that supports target's growth.
Non selective media is reccomended (BAP, Chocolate, TSA)
Collection Container
Appropriate culture media that supports bacterial growth (culture plate/slant)
Packing Instructions
Label samples and ship with cold packs.
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Barron
Tuesday, Wednesday, Thursday, Friday
Results completed in 5-12 days.
WVDL - Madison
Monday, Tuesday, Wednesday, Thursday, Friday
Results completed in 5-12 days.
McMaster's Parasite Quantification- Egg Per Gram (EPG) Test
Useful in determining high egg burdens from bovine, equine, lama, alpaca, caprine and ovine fecal samples.This technique misses low egg output and is not useful as a diagnostic screen. The Wisconsin Sugar Float [LINK] should be used for porcine fecal samples.
Not useful for the recovery of protozoan cysts such as Giardia or oocysts such as Coccidia or Eimeria.
10703 Horses, Bovids, and Camelids including Cattle, Sheep, Goats, Llamas, Alpaca, and Deer PARAQUANT eggs per gram EPG fecal egg count mcmaster mcmaster's mcmasters mc master mc masters mc master's parasite count
Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Mink astrovirus Real Time PCR
Detection of Mink astrovirus by real-time PCR
31498 Mustelids including Ferrets and Mink MASTROPCR shaking mink syndrome neurologic
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Mink enetritis virus Real Time PCR
Detection of Mink enetritis virus by real-time PCR
31511 Mustelids including Ferrets and Mink Carnivore protoparvovirus diarrhea
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Moraxella bovis Real Time PCR
Detection of Moraxella bovis by real-time PCR
31524 Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids MORBOVIS infectious bovine keratoconjunctivitis
Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Moraxella bovoculi Real Time PCR
Detection of Moraxella bovoculi by real-time PCR
31550 Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids MORBOVOCUL infectious bovine keratoconjunctivitis
Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab. Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing. Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Minimum 42 day incubation for liquid culture for bovine samples
Liquid culture can increase the likelihood of detecting very small numbers of Mycobacteria. PCR confirms identification as MAP. No pooling is offered for liquid culture unless samples are from the same animal.
Feces, 2g
If more than 50 samples will be submitted, please call WVDL first to schedule testing prior to collecting the samples.
Species
Cattle
Sampling Instructions
Collect sample aseptically. Do not add formalin or other liquids. Swab must be send in transport media
Collection Container
Sterile, sealed, leak-proof container.
Do not submit in gloves or OB sleeves.
Packing Instructions
Label samples and ship with cold packs.
Special Instructions
Questions regarding herd classification should be directed to the Department of Agriculture, Trade and Consumer Protection's Johne's Disease Control Program at 608-224-4893.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Tuesday, Wednesday, Thursday, Friday
Mycobacterium avium paratuberculosis (MAP)/Johne's Liquid Culture Non - Bovine/Cattle
Minimum 56 day incubation for liquid culture for non-bovine samples.
Liquid culture can increase the likelihood of detecting very small numbers of Mycobacteria. PCR confirms identification as MAP. No pooling is offered for liquid culture unless samples are from the same animal.
10937 Antelope, Gazelle, Goat, Sheep, Llamas, Deer, and other Ruminants
Feces, 2g
If more than 50 samples will be submitted, please call WVDL first to schedule testing prior to collecting the samples.
Species
Antelope, Gazelle, Goat, Sheep, Llamas, Deer, and other Ruminants
Sampling Instructions
Collect sample aseptically. Do not add formalin or other liquids. Swab must be send in transport media
Collection Container
Sterile, sealed, leak-proof container.
Do not submit in gloves or OB sleeves.
Packing Instructions
Label samples and ship with cold packs.
Special Instructions
Questions regarding herd classification should be directed to the Department of Agriculture, Trade and Consumer Protection's Johne's Disease Control Program at 608-224-4893.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Tuesday, Wednesday, Thursday, Friday
Mycobacterium avium paratuberculosis ELISA
The Johne's ELISA is utilized to detect antibodies to Mycobacteriam avium paratuberculosis in bovine, caprine ,ovine sera and bovine milk. This test is primarily intended as a herd test to determine prevalence of Johne's disease. Exotic species can be tested but is not covered under the WVDL's standard operating procedures and will be reported with a disclaimer.
Serum;1 mL minimum. If multiple tests are required, then a 1-2 day delay should be expected. Sending in additional sera samples will remove that delay.
Species
Cattle, Goats, Sheep
Sampling Instructions
Collection Container
Collect serum from clotted blood in a non-additive tube (e.g. plain red top or serum separator). Excessively hemolyzed samples are not acceptable for this test.
Packing Instructions
Refrigerate, ship with cold packs overnight
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
-
Mycobacterium avium paratuberculosis Real Time PCR
Detection of Mycobacterium avium paratuberculosis by real-time PCR (Individual samples)
31563 Cattle, Sheep, Goats, Pigs, Deer, Llamas, Alpacas and other Mammals JOHNEDPCR MAP chronic bacterial enteritis Johnes disease
Cattle, Sheep, Goats, Pigs, Deer, Llamas, Alpacas and other Mammals
Sampling Instructions
Samples should be received by the lab within 7 days of collection. Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hours of test set up.
Mycobacterium avium paratuberculosis Real Time PCR (Multi-dates Pool)
Detection of Mycobacterium avium paratuberculosis by real-time PCR (Pool of multiple dates from same animal)
31576 Cattle, Sheep, Goats, Pigs, Deer, Llamas, Alpacas and other Mammals JOHDPCRPL3 MAP chronic bacterial enteritis Johnes disease
Cattle, Sheep, Goats, Pigs, Deer, Llamas, Alpacas and other Mammals
Sampling Instructions
Samples should be received by the lab within 7 days of collection. Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
-
Results available within 24 hours of test set up.
Mycobacterium avium paratuberculosis Real Time PCR (Pool of 5)
Detection of Mycobacterium avium paratuberculosis by real-time PCR (Pool of up to 5)
31589 Cattle, Sheep, Goats, Pigs, Deer, Llamas, Alpacas and other Mammals JOHDPCRPL MAP chronic bacterial enteritis Johnes disease
Cattle, Sheep, Goats, Pigs, Deer, Llamas, Alpacas and other Mammals
Sampling Instructions
Samples should be received by the lab within 7 days of collection. Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Screening pools can significantly reduce testing costs without compromising test sensitivity. If the pool test yields a positive/inconclusive result, the samples comprising that pool will be tested individually by real-time PCR at an additional cost.
1-3 ml swabs: conjunctival, oral/pharyngeal or tracheal
1g tissues: lung
Species
Birds
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Mycoplasmopsis (Mycoplasma) bovis Real Time PCR
Detection of Mycoplasmopsis (Mycoplasma) bovis by real-time PCR
31615 Cattle, Goats, Sheep, Gazelle, Antelope, other Bovids, and Deer MYCOBOVPCR MBQPCRMLK MBOVPCREYE conjuctivitis pneumonia mastitis
2-3 ml fluids: bronchoalveloar, joint, milk, transtracheal wash
1-3 ml swabs: conjunctival, environmental, joint, oral/pharyngeal, tracheal
1g tissues: brain, lung, lymph node, udder tissue
Species
Cattle, Goats, Sheep, Gazelle, Antelope, other Bovids, and Deer
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Wednesday, Friday
Results available within 24 hours of test set up.
Mycoplasmopsis (Mycoplasma) bovis Real Time PCR (Semen/Embryo)
Detection of Mycoplasmopsis (Mycoplasma) bovis by real-time PCR in semen and embryo wash fluid
31628 Cattle MYCOSEMPCR export regulatory New Zealand
Semen (200uL Extended semen: 4 x 250cc or 2 x 500cc straws)
1 ml embryo wash fluid
Species
Cattle
Sampling Instructions
Semen samples should be sent frozen in liquid nitrogen. Embyro wash fluid may be kept refrigerated until shipping.
Collection Container
Leak-proof tubes or straws.
Packing Instructions
Please utilize a semen tank map and place semen into numerically identified holders that correspond to the paperwork. Bundle semen straws for PCR separately from semen straws for virus isolation or culture testing. Using separate semen tanks for each section is ideal. Embyro wash fluids should be sent in leak-proof containers chilled on ice packs.
Special Instructions
This test is specific for semen and/or embryo samples. We cannot pool semen straws from different collection dates.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Tuesday
Results available within 24 hours of test set up.
Mycoplasmopsis (Mycoplasma) Culture
Culture for Mycolpasmosis species on selective media.
A result of growth indicates Mycoplasma and/or Acholeplasma species grew in bacteriological culture. This test does not distinguish between these bacteria.
Fresh respiratory fluid, lung tissue, swab is transport media, or raw semen
Porcine samples will be referred for testing.
Species
Sampling Instructions
Collect sample aseptically. Do not add formalin or other liquids.
Collection Container
Sterile, sealed, leak-proof container
Packing Instructions
Label samples and ship with cold packs.
Special Instructions
Swabs must be in transport media.
Results should be interpreted in the context of all available clinical, historical, and epidemiological information.
Further testing options including utilizing the Mycoplasmopsis bovis PCR[LINK], which can be performed from this culture.
Mycoplasma ovipneumoniae, primarily a pathogen of sheep, will not grow on the media used for this culture. Referral for PCR is recommended.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Tuesday, Wednesday, Thursday, Friday
Results completed in 5-12 days.
WVDL - Barron
Monday, Tuesday, Wednesday, Thursday, Friday
Results completed in 5-12 days.
Mycoplasmopsis (Mycoplasma) Culture from Milk Sample
Culture for Mycolpasmosis species on selective media.
A result of growth indicates Mycoplasma and/or Acholeplasma species grew in bacteriological culture. This test does not distinguish between these bacteria.
10651 MYCOCULTMK Mycoplasmopsis Mycoplasmoides milk bulk tank milk
Collect sample aseptically. Do not add formalin or other liquids.
Collection Container
Sterile, sealed, leak-proof container
Packing Instructions
Label samples and ship with cold packs.
Special Instructions
Results should be interpreted in the context of all available clinical, historical, and epidemiological information.
Further testing options including utilizing the Mycoplasmopsis bovis PCR[LINK], which can be performed from this culture.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Barron
Monday, Tuesday, Wednesday, Thursday, Friday
Results completed in 5-12 days.
WVDL - Madison
Monday, Tuesday, Wednesday, Thursday, Friday
Results completed in 5-12 days.
Mycoplasmopsis (Mycoplasma) Culture from Pooled Milk Sample
Culture for Mycolpasmosis species on selective media.
A result of growth indicates Mycoplasma and/or Acholeplasma species grew in bacteriological culture. This test does not distinguish between these bacteria.
10664 MYCOCULTPL Mycoplasmopsis Mycoplasmoides milk pool pooled samples pooled milk
Milk samples only. Up to 5 samples per pool. If pool grows mycoplasma or is contaminated, individual samples are cultured.
Species
Sampling Instructions
Collect sample aseptically. Do not add formalin or other liquids.
Collection Container
Sterile, sealed, leak-proof container
Packing Instructions
Label samples and ship with cold packs.
Special Instructions
Incubation period for culture is one week. Re-culturing individual milks that were in a contaminated or positive pool requires an additional week of culture.
Results should be interpreted in the context of all available clinical, historical, and epidemiological information.
Further testing options including utilizing the Mycoplasmopsis bovis PCR[LINK], which can be performed from this culture.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Tuesday, Wednesday, Thursday, Friday
Results completed in 5-12 days.
WVDL - Barron
Monday, Tuesday, Wednesday, Thursday, Friday
Results completed in 5-12 days.
Mycoplasmopsis (Mycoplasma) Culture from Semen
Culture for Mycolpasmosis species on selective media.
A result of growth indicates Mycoplasma and/or Acholeplasma species grew in bacteriological culture. This test does not distinguish between these bacteria.
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Mycoplasmopsis (Mycoplasma) felis Real Time PCR
Detection of Mycoplasmopsis (Mycoplasma) felis by real-time PCR
1-3 ml swabs: conjunctival, nasal, oral/pharyngeal, tracheal
2-3 ml bronchoalveloar fluid
1g tissues: brain, lung
Species
Cats
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Mycoplasmopsis (Mycoplasma) synoviae ELISA
Can't find this ELISA by itself (can only find in Panels 1&2)
Serum 0.5 ml; 1 ml if additional tests are requested.
Species
Chicken, Turkey
Sampling Instructions
HI test is run to confirm plate or ELISA test positive results. NOTE, currently, the MS HI for turkey sera is not available at the WVDL and will be referred.
Collection Container
Packing Instructions
Refrigerate; ship with cold packs. Do not freeze.
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Barron
-
Mycoplasmopsis (Mycoplasma) synoviae Real Time PCR
Detection of Mycoplasmopsis (Mycoplasma) synoviae by real-time PCR
31745 Birds AMYSYNPCR infectious synovitis avian mycoplasmpsis mycoplasma arthritis MS
1-3 ml swabs: conjunctival, oral/pharyngeal or tracheal
1g tissues: lung
Species
Birds
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Any age bovine, sheep, goat, cervid, llama, alpaca, pig, mink, poultry, commercial fish. For more information, see Pathology Price Sheet under Test and Fees link on WVDL website.
Species
Sampling Instructions
Collection Container
Packing Instructions
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
-
WVDL - Barron
-
Necropsy – Non-Production Animal (101-500 lb)
Non-production animal necropsy on an animal weighing between 101 and 500lb.
Any age horse, dog, cat, pocket pet, pet bird, zoo animal, wildlife - 101-500lb body weight. For more information, see Pathology Price Sheet under Test and Fees link on WVDL website.
Species
Sampling Instructions
Collection Container
Packing Instructions
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
-
WVDL - Barron
-
Necropsy – Non-Production Animal (5-100 lb)
Non-production animal necropsy on an animal weighing between 5 and100lb.
Any age horse, dog, cat, pocket pet, pet bird, zoo animal, wildlife - 5-100 lb body weight. For more information, see Pathology Price Sheet under Test and Fees link on WVDL website.
Species
Sampling Instructions
Collection Container
Packing Instructions
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
-
WVDL - Barron
-
Necropsy – Non-Production Animal (<5 lb)
Non-production animal necropsy on an animal weighing less than 5 pounds.
Any age horse, dog, cat, pocket pet, pet bird, zoo animal, wildlife less than 5lb body weight. For more information, see Pathology Price Sheet under Test and Fees link on WVDL website.
Species
Sampling Instructions
Collection Container
Packing Instructions
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
-
WVDL - Barron
-
Necropsy – Non-Production Animal (>500 lb)
Non-production animal necropsy on an animal weighing more than 500lb.
Any age horse, dog, cat, pocket pet, pet bird, zoo animal, wildlife more than 500 lb body weight. For more information, see Pathology Price Sheet under Test and Fees link on WVDL website.
Species
Sampling Instructions
Collection Container
Packing Instructions
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
-
WVDL - Barron
-
Neorickettsia risticii Real Time PCR
Detection of Neorickettsia risticii by real-time PCR
31758 Equines, including Horses, Donkeys, Mules, and Zebras NEORICKPCR Erlichia Potomac horse fever colitis enteric diarrhea
Equines, including Horses, Donkeys, Mules, and Zebras
Sampling Instructions
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Neospora caninum Competitive ELISA
The Neospora caninum cELISA is utilized to detect antibody to N. caninum in bovine and caprine sera
1-3 mL serum or 3-5 mLs clotted blood. If requesting 3 or more serological assays, please submit 10 mLs serum to accommodate all tests requested.
Species
Cattle
Sampling Instructions
Collection Container
Collect serum from clotted blood in a non-additive tube (e.g. plain red top or serum separator). Excessively hemolyzed samples are not acceptable for this test.
Packing Instructions
Refrigerate, ship with cold packs overnight
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
-
Neospora caninum Real Time PCR
Detection of Neospora caninum by real-time PCR
31771 Cattle, Goats, Sheep, Gazelle, Antelope,other Bovids, Dogs, and Camelids including Llamas and Alpacas NEOPCR coccidian parasite
Cattle, Goats, Sheep, Gazelle, Antelope,other Bovids, Dogs, and Camelids including Llamas and Alpacas
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab. Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing. Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Package placenta in a separately than other samples.
Label all samples.
Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids
Sampling Instructions
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
If an FAD (Foreign Animal Disease) differential is suspected, contact the AVIC and DATCP prior to WVDL submission.
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Osmolality Of Milk, Milk Replacer And Electrolyte Solutions Via Freeze Depression Analysis For Submissions Of 1 To 5 Samples.
Osmolality of milk, milk replacer and electrolyte solutions via freeze depression analysis for submissions of 1 to 5 samples.
Milk, milk replacer or electrolyte solutions. Modifications to the liquid feed, such as the addition of probiotics, has not been validated for this assay and is not covered under our standard operating procedures. If submitting liquid feed that might have modifications, the use of these results for any purpose is at the discretion of the submitter. Please contact the WVDL with any questions.
Species
Sampling Instructions
Within the dairy industry, calves are given milk replacer to provide protein, fats, carbohydrates, and nutrients in place of the mother¿s milk. The ratio of powder to water in mixing milk replacer is very important as high concentrations can lead to delayed nutrient absorption, diarrhea, bloat, and sodium toxicity. Osmolality, the total solute concentration of an aqueous solution, is used as an indicator of proper milk replacer to water ratio. Coupled with total solids, an osmolality reading can help determine if a farm is using the proper amount, or kind, of milk replacer for their calves.
Collection Container
Packing Instructions
Samples must be sent in 2 or 3oz snap-top vials and kept refrigerated at 0-6°C. Visit our website and click on Submission Guidelines/Forms/Veterinary Supply Order Form to purchase milk vials.
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Barron
-
Osmolality Of Milk, Milk Replacer And Electrolyte Solutions Via Freeze Depression Analysis For Submissions Of Greater Than 5 Samples.
Osmolality of milk, milk replacer and electrolyte solutions via freeze depression analysis for submissions of 1 to 5 samples.
Milk, milk replacer or electrolyte solutions. Modifications to the liquid feed, such as the addition of probiotics, has not been validated for this assay and is not covered under our standard operating procedures. If submitting liquid feed that might have modifications, the use of these results for any purpose is at the discretion of the submitter. Please contact the WVDL with any questions.
Species
Sampling Instructions
Within the dairy industry, calves are given milk replacer to provide protein, fats, carbohydrates, and nutrients in place of the mother¿s milk. The ratio of powder to water in mixing milk replacer is very important as high concentrations can lead to delayed nutrient absorption, diarrhea, bloat, and sodium toxicity. Osmolality, the total solute concentration of an aqueous solution, is used as an indicator of proper milk replacer to water ratio. Coupled with total solids, an osmolality reading can help determine if a farm is using the proper amount, or kind, of milk replacer for their calves.
Collection Container
Packing Instructions
Samples must be sent in 2 or 3oz snap-top vials and kept refrigerated at 0-6°C. Visit our website and click on Submission Guidelines/Forms/Veterinary Supply Order Form to purchase milk vials.
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Barron
-
Ovine herpesvirus Type 2 Real Time PCR
Detection of Ovine herpesvirus Type 2 by real-time PCR
31797 Cattle, Goats, Sheep, Gazelle, Antelope, other Bovids, Pigs, and Deer MCFPCR malignant catarrhal fever MCF OvHV-2
Cattle, Goats, Sheep, Gazelle, Antelope, other Bovids, Pigs, and Deer
Sampling Instructions
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Wednesday, Friday
Results available within 24 hours of test set up.
Parasite Quantification On Feces using Wisconsin Sugar Flotation Method
Recovery of relatively small amounts of helminth ova. Useful for checking the effectiveness of a dewormer drug program when pre and post deworming samples are taken.
Not useful for the recovery of protozoan cysts such as Giardia or oocysts such as Coccidia or Eimeria.
10716 PARAQUANTS eggs per 5 grams ep5g parasite count egg count WI sugar float WI sugar flotation Wisconsin Sugar Float
Birds, Cattle, Goats, Sheep, Gazelle, Antelope, other Bovids, Deer, and Pigs
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Porcine (Swine) Abortion Culture Panel
Culture for aerobic bacteria known to cause abortions including Listeria and Salmonella
Mycoplasmopsis and Ureaplamsa not included.
Collect sample aseptically. Do not add formalin or other liquids.
Collection Container
Sterile, sealed, leak-proof container
Packing Instructions
Label samples and ship with cold packs.
Special Instructions
Swabs must be in transport media
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Barron
Monday, Tuesday, Wednesday, Thursday, Friday
Results completed in 5-12 days.
WVDL - Madison
Monday, Tuesday, Wednesday, Thursday, Friday
Results completed in 5-12 days.
Porcine Abortion PCR Panel
Detection of pathogens commonly attributed to abortion in porcine; includes Porcine parvovirus, Porcine circovirus type 2, and Porcine Respiratory Reproductive Syndrome by real-time PCR
1g tissue: intestine, lung, placenta, spleen, or fetal tissue
Species
Pigs
Sampling Instructions
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Porcine circovirus Type 2 Real Time PCR
Detection of Porcine circovirus Type 2 by real-time PCR
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Porcine Enteric PCR Panel
Detection of pathogens commonly attributed to enteric disease in porcine; includes Porcine parvovirus (PPV), Lawsonia intracellularis, Transmissible gastroenteritis virus (TGE), Porcine epidemic diarrhea virus (PEDV), and Swine deltacoronavirus (SDCoV) by real-time PCR.
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Collect sample aseptically. Do not add formalin or other liquids. Swab must be send in transport media
Collection Container
Sterile, sealed, leak-proof container
Packing Instructions
Label samples and ship with cold packs.
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Barron
Monday, Tuesday, Wednesday, Thursday, Friday
Results completed in 5-12 days.
WVDL - Madison
Monday, Tuesday, Wednesday, Thursday, Friday
Results completed in 5-12 days.
Porcine Respiratory PCR Panel
Detection of pathogens commonly attributed to respiratory disease in porcine; includes Porcine circovirus Type 2, Influenza A and Porcine reproductive and respiratory syndrome by real-time PCR
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Porcine respiratory reproductive syndrome virus Real Time PCR
Detection of Porcine respiratory reproductive syndrome by real-time PCR
31888 Pigs PRRS.PCR PRRSINDPCR PRRS blue ear disease reproductive respiratory SWD swine infertility and respiratory syndrome SIRS
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Wednesday, Thursday, Friday
Results available within 24 hours of test set up.
Porcine respiratory reproductive syndrome virus Real Time PCR (Pool of 3)
Detection of Porcine Respiratory Reproductive Syndrome real-time PCR (Pool of 3)
31901 Pigs PRRSPCRPL3 PRRS blue ear disease reproductive respiratory SWD swine infertility and respiratory syndrome SIRS
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Screening pools can significantly reduce testing costs without compromising test sensitivity. If the pool test yields a positive/inconclusive result, the samples comprising that pool will be tested individually by real-time PCR at an additional cost.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Wednesday, Thursday, Friday
Results available same day.
Porcine respiratory reproductive syndrome virus Real Time PCR (Pool of 5)
Detection of Porcine Respiratory Reproductive Syndrome real-time PCR (Pool of 5)
31914 Pigs PRRSPCRPL PRRS blue ear disease reproductive respiratory SWD swine infertility and respiratory syndrome SIRS
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Screening pools can significantly reduce testing costs without compromising test sensitivity. If the pool test yields a positive/inconclusive result, the samples comprising that pool will be tested individually by real-time PCR at an additional cost.
Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Prototheca Culture
Quantified culture of Prototheca species in milk or water samples
10781 PROTOTHECA Prototheca culture Proto culture Prototheca zopfii mastitisis prototheca isolatation media
Milk or water sample, 5 mL; Bedding material, at minimum 2 g
Species
Sampling Instructions
Collect sample aseptically. Do not add formalin or other liquids.
Collection Container
Sterile, sealed, leak-proof container
Packing Instructions
Label samples and ship with cold packs. DO NOT FREEZE
Special Instructions
Can be added to a Mastitis and/or Mycoplasma milk culture. Bulk tank culture already has Prototheca culture within this testin procedure.
Prototheca found in water may be below the level of detection for this test. Genotyping can be performed upon request.
NOTE: Internal data demonstrates a significant loss of recovery of Prototheca when the sample is frozen. If the sample was frozen, the use of those results for any purpose is at the discretion of the submitter.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Barron
Monday, Tuesday, Wednesday, Thursday, Friday
Results completed in 5-12 days.
WVDL - Madison
Monday, Tuesday, Wednesday, Thursday, Friday
Results completed in 5-12 days.
Psittacine herpesvirus Type 1 Real Time PCR
Detection of Psittacine herpesvirus Type 1 by real-time PCR
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Renibacterium salmoninarum Conventional PCR
Detection of Renibacterium salmoninarum by conventional PCR
31953 Aquatic Species RSAL-PCR Corynebacterium BKD bacterial kidney disease
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
-
As needed
Renibacterium salmoninarum Culture
Aerobic culture on bacteriologic media for Renibacterium salmoninarum
Equines, including Horses, Donkeys, Mules, and Zebras
Sampling Instructions
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Fresh tissue, fluid, or swab in bacterial transport media
Species
Sampling Instructions
Collect sample aseptically. Do not add formalin or other liquids. Swab must be send in transport media
Collection Container
Sterile, sealed, leak-proof container
Packing Instructions
Label samples and ship with cold packs.
Special Instructions
Seroptying[LINK] is automatically performed, unless declined on submission form.
An additional charge is added for serotyping [LINK] and susceptibility testing.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Barron
Monday, Tuesday, Wednesday, Thursday, Friday
Results completed in 5-12 days.
WVDL - Madison
Monday, Tuesday, Wednesday, Thursday, Friday
Results completed in 5-12 days.
Salmonella Culture From Diagnostic-Related, Non-Biological Samples
This is a diagnostic-related salmonella test on non-biological specimens and is NOT intended for premise or product certification/analysis for any federal, state or local entity.
10846 SALMNONBIO nonbio Salmonella species Salmonellosis drag swab SE Environmental swab Envrionment
Bovine serum; 1 mL minimum, Bovine milk, bulk milk; 5 mL
Species
Cattle
Sampling Instructions
Collection Container
Collect serum from clotted blood in a non-additive tube (e.g. plain red top or serum separator). Excessively hemolyzed samples are not acceptable for this test.
Packing Instructions
Refrigerate, ship with cold packs overnight
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
-
Salmonella O Grouping
Salmonella species O grouping
10859 SALMGROUP salmonella serotyping Salm serotyping salmonella O group Salm O group
Cattle, Goats, Sheep, Deer, Pigs, Dogs, Cats, Birds, and Horses
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
All Salmonella spp. PCR results that yield a CT value of less than or equal to 35, will automatically be sent for culture and serotyping. If you do NOT want these additional tests performed or only want one of these tests performed, please contact the WVDL immediately. For more information please see our website for a full description of our Salmonella workflow.
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Swab in 1-3 ml media (VTM, BHI, PBS) 1 g fresh tissue
Species
Cats, Dogs, and Mustelids including Mink and Ferrets
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab. Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing. Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
-
As Needed
SARS-CoV-2 Surrogate Viral Neutralization Test
Detection of antibodies to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by Surrogate Viral Neutralization Test.
40030 Cats, Dogs SARS COVID Severe Acute Respiratory Syndrrome CoV-2 Coronavirus SARSCOV2VN Cat Dog
Collect serum from clotted blood in a non-additive tube. Avoid hemolysis.
Collection Container
Serum samples should be submitted in serum separator tubes, uncoated/plain tubes may be appropriate for spun serum.
Packing Instructions
Label all samples with unique identifiers. Ship overnight on cold packs.
Special Instructions
The SARS-CoV-2 Surrogate Virus Neutralization Test Kit (“sVNT”) is a functional Enzyme-Linked Immunosorbent Assay (ELISA) This kit is not USDA licensed. Please contact WVDL Virology section prior to submission.
Submit obex and half of each retropharyngeal lymph node in a leak proof jar containing 10% neutral buffered formalin.
Species
Sheep, Goats
Sampling Instructions
Formalin fixed tissue
Collection Container
Leak-proof jar containing 10% neutral buffered formalin at a ratio of 10 times the volume of tissue.
Packing Instructions
Each container must be labeled with the official identification that is human readable (minimum) and a barcode (best practice) on the side of the container. Additionally, the top of the jar must be labeled with the position number of the associated submission.
Retropharyngeal lymph node in a leak proof jar containing 10% neutral buffered formalin.
Species
Sheep, Goats
Sampling Instructions
Leak-proof jar containing 10% neutral buffered formalin at a ratio of 10 times the volume of tissue.
Collection Container
Each container must be labeled with the official identification that is human readable (minimum) and a barcode (best practice) on the side of the container. Additionally, the top of the jar must be labeled with the position number of the associated submission.
Packing Instructions
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Tuesday, Wednesday, Thursday, Friday
Within 10 business days.
Semen Virus Isolation- Bovine herpesvirus Type 1 (1 pass)
Isolation and identification of bovine herpesvirus 1 (BHV-1) using cell culture (1 pass).
Raw semen: 100uL Extended semen: 4 x 250cc or 2 x 500cc straws.
Species
Cattle
Sampling Instructions
Samples should be sent frozen in liquid nitrogen.
Collection Container
Leak-proof tubes or straws.
Packing Instructions
Please utilize a semen tank map and place semen into numerically identified holders that correspond to the paperwork. Bundle semen straws for virus isoaltion separately from semen straws for PCR testing. Using separate semen tanks for each section is ideal.
Special Instructions
Turn-around time will be affected by a drastic increase in requests for an individual test, arrival time of the specimen at the laboratory and poor quality samples that will require additional processing. Advanced scheduling is REQUIRED when submitting greater than 20 semen samples at one time. Please contact the Virology Section at least one week prior to submission.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Tuesday, Wednesday, Thursday, Friday
Results completed within 10-12 days
Semen Virus Isolation- Bovine herpesvirus Type 1 (2 pass)
Isolation and identification of bovine herpesvirus 1 (BHV-1) using cell culture (2 pass).
Raw semen: 100uL Extended semen: 4 x 250cc or 2 x 500cc straws.
Species
Cattle
Sampling Instructions
Samples should be sent frozen in liquid nitrogen.
Collection Container
Leak-proof tubes or straws.
Packing Instructions
Please utilize a semen tank map and place semen into numerically identified holders that correspond to the paperwork. Bundle semen straws for virus isoaltion separately from semen straws for PCR testing. Using separate semen tanks for each section is ideal.
Special Instructions
Turn-around time will be affected by a drastic increase in requests for an individual test, arrival time of the specimen at the laboratory and poor quality samples that will require additional processing. Advanced scheduling is REQUIRED when submitting greater than 20 semen samples at one time. Please contact the Virology Section at least one week prior to submission.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Tuesday, Wednesday, Thursday, Friday
Results completed within 14-19 days
Semen Virus Isolation- Bovine herpesvirus Type 1 (3 pass)
Isolation and identification of bovine herpesvirus 1 (BHV-1) using cell culture (3 pass).
Raw semen: 100uL Extended semen: 4 x 250cc or 2 x 500cc straws.
Species
Cattle
Sampling Instructions
Samples should be sent frozen in liquid nitrogen.
Collection Container
Leak-proof tubes or straws.
Packing Instructions
Please utilize a semen tank map and place semen into numerically identified holders that correspond to the paperwork. Bundle semen straws for virus isoaltion separately from semen straws for PCR testing. Using separate semen tanks for each section is ideal.
Special Instructions
Turn-around time will be affected by a drastic increase in requests for an individual test, arrival time of the specimen at the laboratory and poor quality samples that will require additional processing. Advanced scheduling is REQUIRED when submitting greater than 20 semen samples at one time. Please contact the Virology Section at least one week prior to submission.
Isolation and identification of bovine viral diarrhea virus (BVDV) (1 pass) and bovine herpesvirus 1 (BHV-1) using cell culture (1 pass) and BVD PCR confirmation.
Raw semen: 100uL Extended semen: 4 x 250cc or 2 x 500cc straws.
Species
Cattle
Sampling Instructions
Samples should be sent frozen in liquid nitrogen.
Collection Container
Leak-proof tubes or straws.
Packing Instructions
Please utilize a semen tank map and place semen into numerically identified holders that correspond to the paperwork. Bundle semen straws for virus isoaltion separately from semen straws for PCR testing. Using separate semen tanks for each section is ideal.
Special Instructions
Turn-around time will be affected by a drastic increase in requests for an individual test, arrival time of the specimen at the laboratory and poor quality samples that will require additional processing. Advanced scheduling is REQUIRED when submitting greater than 20 semen samples at one time. Please contact the Virology Section at least one week prior to submission.
Isolation and identification of bovine viral diarrhea virus (BVDV) (1 pass) and bovine herpesvirus 1 (BHV-1) (2 pass) using cell culture and BVD PCR confirmation.
Raw semen: 100uL Extended semen: 4 x 250cc or 2 x 500cc straws.
Species
Cattle
Sampling Instructions
Samples should be sent frozen in liquid nitrogen.
Collection Container
Leak-proof tubes or straws.
Packing Instructions
Please utilize a semen tank map and place semen into numerically identified holders that correspond to the paperwork. Bundle semen straws for virus isoaltion separately from semen straws for PCR testing. Using separate semen tanks for each section is ideal.
Special Instructions
Turn-around time will be affected by a drastic increase in requests for an individual test, arrival time of the specimen at the laboratory and poor quality samples that will require additional processing. Advanced scheduling is REQUIRED when submitting greater than 20 semen samples at one time. Please contact the Virology Section at least one week prior to submission.
Isolation and identification of bovine viral diarrhea virus (BVDV) (1 pass) and bovine herpesvirus 1 (BHV-1) (3 pass) using cell culture and BVD PCR confirmation.
Raw semen: 100uL Extended semen: 4 x 250cc or 2 x 500cc straws.
Species
Cattle
Sampling Instructions
Samples should be sent frozen in liquid nitrogen.
Collection Container
Leak-proof tubes or straws.
Packing Instructions
Please utilize a semen tank map and place semen into numerically identified holders that correspond to the paperwork. Bundle semen straws for virus isoaltion separately from semen straws for PCR testing. Using separate semen tanks for each section is ideal.
Special Instructions
Turn-around time will be affected by a drastic increase in requests for an individual test, arrival time of the specimen at the laboratory and poor quality samples that will require additional processing. Advanced scheduling is REQUIRED when submitting greater than 20 semen samples at one time. Please contact the Virology Section at least one week prior to submission.
Raw semen: 100uL Extended semen: 4 x 250cc or 2 x 500cc straws.
Species
Cattle
Sampling Instructions
Samples should be sent frozen in liquid nitrogen.
Collection Container
Leak-proof tubes or straws.
Packing Instructions
Please utilize a semen tank map and place semen into numerically identified holders that correspond to the paperwork. Bundle semen straws for virus isoaltion separately from semen straws for PCR testing. Using separate semen tanks for each section is ideal.
Special Instructions
Turn-around time will be affected by a drastic increase in requests for an individual test, arrival time of the specimen at the laboratory and poor quality samples that will require additional processing. Advanced scheduling is REQUIRED when submitting greater than 20 semen samples at one time. Please contact the Virology Section at least one week prior to submission.
Isolation and identification of bovine viral diarrhea virus (BVDV) (2 pass) and bovine herpesvirus 1 (BHV-1) 1 pass) using cell culture and BVD PCR confirmation.
Raw semen: 100uL Extended semen: 4 x 250cc or 2 x 500cc straws.
Species
Cattle
Sampling Instructions
Samples should be sent frozen in liquid nitrogen.
Collection Container
Leak-proof tubes or straws.
Packing Instructions
Please utilize a semen tank map and place semen into numerically identified holders that correspond to the paperwork. Bundle semen straws for virus isoaltion separately from semen straws for PCR testing. Using separate semen tanks for each section is ideal.
Special Instructions
Turn-around time will be affected by a drastic increase in requests for an individual test, arrival time of the specimen at the laboratory and poor quality samples that will require additional processing. Advanced scheduling is REQUIRED when submitting greater than 20 semen samples at one time. Please contact the Virology Section at least one week prior to submission.
Isolation and identification of bovine viral diarrhea virus (BVDV) (2 pass) and bovine herpesvirus 1 (BHV-1) (2 pass) using cell culture and BVD PCR confirmation.
Raw semen: 100uL Extended semen: 4 x 250cc or 2 x 500cc straws.
Species
Cattle
Sampling Instructions
Samples should be sent frozen in liquid nitrogen.
Collection Container
Leak-proof tubes or straws.
Packing Instructions
Please utilize a semen tank map and place semen into numerically identified holders that correspond to the paperwork. Bundle semen straws for virus isoaltion separately from semen straws for PCR testing. Using separate semen tanks for each section is ideal.
Special Instructions
Turn-around time will be affected by a drastic increase in requests for an individual test, arrival time of the specimen at the laboratory and poor quality samples that will require additional processing. Advanced scheduling is REQUIRED when submitting greater than 20 semen samples at one time. Please contact the Virology Section at least one week prior to submission.
Isolation and identification of bovine viral diarrhea virus (BVDV) (2 pass) and bovine herpesvirus 1 (BHV-1) (3 pass) using cell culture and BVD PCR confirmation.
Raw semen: 100uL Extended semen: 4 x 250cc or 2 x 500cc straws.
Species
Cattle
Sampling Instructions
Samples should be sent frozen in liquid nitrogen.
Collection Container
Leak-proof tubes or straws.
Packing Instructions
Please utilize a semen tank map and place semen into numerically identified holders that correspond to the paperwork. Bundle semen straws for virus isoaltion separately from semen straws for PCR testing. Using separate semen tanks for each section is ideal.
Special Instructions
Turn-around time will be affected by a drastic increase in requests for an individual test, arrival time of the specimen at the laboratory and poor quality samples that will require additional processing. Advanced scheduling is REQUIRED when submitting greater than 20 semen samples at one time. Please contact the Virology Section at least one week prior to submission.
Raw semen: 100uL Extended semen: 4 x 250cc or 2 x 500cc straws.
Species
Cattle
Sampling Instructions
Samples should be sent frozen in liquid nitrogen.
Collection Container
Leak-proof tubes or straws.
Packing Instructions
Please utilize a semen tank map and place semen into numerically identified holders that correspond to the paperwork. Bundle semen straws for virus isoaltion separately from semen straws for PCR testing. Using separate semen tanks for each section is ideal.
Special Instructions
Turn-around time will be affected by a drastic increase in requests for an individual test, arrival time of the specimen at the laboratory and poor quality samples that will require additional processing. Advanced scheduling is REQUIRED when submitting greater than 20 semen samples at one time. Please contact the Virology Section at least one week prior to submission.
Raw semen: 100uL Extended semen: 4 x 250cc or 2 x 500cc straws.
Species
Cattle
Sampling Instructions
Samples should be sent frozen in liquid nitrogen.
Collection Container
Leak-proof tubes or straws.
Packing Instructions
Please utilize a semen tank map and place semen into numerically identified holders that correspond to the paperwork. Bundle semen straws for virus isoaltion separately from semen straws for PCR testing. Using separate semen tanks for each section is ideal.
Special Instructions
Turn-around time will be affected by a drastic increase in requests for an individual test, arrival time of the specimen at the laboratory and poor quality samples that will require additional processing. Advanced scheduling is REQUIRED when submitting greater than 20 semen samples at one time. Please contact the Virology Section at least one week prior to submission.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Tuesday, Wednesday, Thursday, Friday
Results completed within 16-22 days
Senecavirus A Real Time PCR (Pool)
Detection of Senecavirus A by real-time PCR (Pool of 5)
32018 Pigs SVAPCRPL vesicular disease SVA Senecavirus PCR Seneca Valley virus
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Screening pools can significantly reduce testing costs without compromising test sensitivity. If the pool test yields a positive/inconclusive result, the samples comprising that pool will be tested individually by real-time PCR at an additional cost.
Collect serum from clotted blood in a non-additive tube (e.g. plain red top or serum separator). Avoid hemolysis. Fresh serum samples must be received at WVDL within 7 days of bleed date.
Collection Container
Serum samples should be submitted in serum separator tubes.
Packing Instructions
Label all samples with unique identifiers. Ship overnight on cold packs.
Special Instructions
Serum should be used for adult animals and calves > 6 months of age. Turn-around time will be affected by a drastic increase in requests for an individual test, arrival time of the specimen at the laboratory and poor quality samples that will require additional processing. Advanced scheduling is REQUIRED when submitting greater than 20 samples at one time. Please contact the Virology Section at least one week prior to submission.
Collect serum from clotted blood in a non-additive tube (e.g. plain red top or serum separator). Avoid hemolysis. Fresh serum samples must be received at WVDL within 7 days of bleed date.
Collection Container
Serum samples should be submitted in serum separator tubes.
Packing Instructions
Label all samples with unique identifiers. Ship overnight on cold packs.
Special Instructions
Turn-around time will be affected by a drastic increase in requests for an individual test, arrival time of the specimen at the laboratory and poor quality samples that will require additional processing. Advanced scheduling is REQUIRED when submitting greater than 20 samples at one time. Please contact the Virology Section at least one week prior to submission.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Wednesday, Friday
Results competed in 10-14 days
Small Ruminant Abortion PCR Panel
Detection of pathogens commonly attributed to abortion in small ruminants; includes Chlamydia spp, Campylobacter jejuni, Coxiella burnetii, Leptospira spp, Neospora caninum and Toxoplasma gondii by real-time PCR
32083 Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids ABORPNLRUM CHLAM LEPTO QFEVER QF NEO TOXO Campy jejuni
Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids
Sampling Instructions
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Package placenta separately from other tissues.
Label all samples.
Ship overnight on cold packs in an insulated container.
Special Instructions
Samples being test for Coxiella are processed in a BSL3 laboratory, this may affect test turnaround times.
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Small Ruminant Lentivirus (OPP/CAE) Antibody cELISA
Collect serum from clotted blood in a non-additive tube (e.g. plain red top or serum separator). Excessively hemolyzed samples are not acceptable for this test.
Collection Container
Non-additive tube
Packing Instructions
Refrigerate; ship with cold packs
Special Instructions
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Barron
Wednesday
Generally completed on Wednesday. Results available same day.
Small Ruminant Mimic Panel
Detection of Ovine herpesviurs Type 2 (OvHV-2, Malignant catarrhal fever (MCF)), Bluetongue virus, Epizootic hemorrhagic disease virus and ORF virus by real-time PCR
31537 Bovids, including Cattle, Sheep, Goats, Antelope, and Gazelle MCF, ORF, BTV, EHD
Bovids, including Cattle, Sheep, Goats, Antelope, and Gazelle
Sampling Instructions
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
***If a FAD (Foreign Animal Disease) differential is suspected, contact the AVIC and DATCP prior to WVDL submission.*** Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up
Small Ruminant Respiratory PCR Panel
Detection of pathogens commonly attributed to respiratory disease in small ruminants; includes Chlamydia spp, Mannheimia haemolytica, Pasteurella multocida, Histophilus somni, Mycoplasmopsis bovis, Bibersteinia trehalosi and Bovine respiratory syncytial virus (BRSV) by real-time PCR
Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Streptococcus equi equi Real Time PCR
Detection of Streptococcus equi equi by real-time PCR
32109 Equines, including Horses, Donkeys, Mules, and Zebras SEQUI.PCR Strangles respiratory
Equines, including Horses, Donkeys, Mules, and Zebras
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Streptococcus equi zooepidemicus Real Time PCR
Detection of Streptococcus equi zooepidemicus by real-time PCR
32122 Dogs and Equines including Horses, Donkeys, Mules, and Zebras SZOO.PCR respiratory pneumonia
Dogs and Equines including Horses, Donkeys, Mules, and Zebras
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab. Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing. Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
SVA/FMDV multiplex Real Time PCR
Detection of Senecavirus A (SVA) and foot and mouth disease (FMD) virus by multiplexing real time PCR.
32317 Cattle, Sheep, Goats, Pigs, Camelids, Cervids NAHLN, FMDSVAMPX,FMDVPCR, FMDMPX, FMD, FMDV, Foot and mouth disease virus, PCR, hoof and mouth SVA, Senecavirus, PCR, Seneca Valley virus, multiplex
Vesicular tissue, oral or lesion swab in tris-buffered tryptose broth (TBTB). Swabs should be submitted in a minimum of 3 ml TBTB.
Species
Cattle, Sheep, Goats, Pigs, Camelids, Cervids
Sampling Instructions
Swabs should be submitted in TBTB. Swabs must be removed from media. Swirl each swab in the liquid, press the tip of the swab on the side of the tube to release remaining liquid.
Collection Container
Swabs or tissues should be submitted in a labeled, sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs to arrive within 48 hours of collection.
Special Instructions
NAHLN assay. Test is performed for Foreign Animal Disease (FAD) investigations. Contact AVIC and DATCP prior to WVDL submission. Non-Negative results are confirmed at NVSL-FADDL.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
-
As needed. TAT will be based on FADI priority level.
Swine Enteric Coronavirus Disease Multiplex Real Time PCR Panel
Detection of Swine enteric coronavirus panel; includes porcine epidemic diarrhea virus (PEDV), Transmissible gastroenteritis virus (TGEV), and Swine deltacoronavirus (SDCoV) by real-time PCR
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Wednesday, Thursday, Friday
Results available within 24 hours of test set up.
Swine Enteric Coronavirus Disease Multiplex Real Time PCR Panel (Same day testing)
Detection of Swine enteric coronavirus panel; includes porcine epidemic diarrhea virus (PEDV), Transmissible gastroenteritis virus (TGEV), and Swine deltacoronavirus (SDCoV) by real-time PCR. Results are available same day.
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Wednesday, Thursday, Friday
Results available same day.
Tissue Harvest of Commerical Poultry Less Than 3 Days Of Age For Salmonella Culture Only
Cattle, Goats, Sheep, Gazelle, Antelope, other Bovids, Cats, and Dogs,
Sampling Instructions
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Tritrichomonas foetus Culture
10911 Bovids and Cats TRICH-CULT Trich Trich foetus trichomonas tritrichomonas foetus Trichomonas test Cattle bull
Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
2 In-pouches are required if PCR and culture are requested.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Wednesday, Friday
Results available within 24 hours of test set up.
Turkey ELISA Panel: BA, HEV and NDV ELISAs
BA HE NDV Turkey ELISA Panel
20145 Turkey AVIAN SEROLOGY ELISA BACTERIA BORDATELLA AVIUM NEW CASTLE VIRUS HEMORRHAGIC ENTERITIS HE BA NDV BART
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Tuesday, Thursday
Results available within 24 hour of test scheduled set-up.
Cattle, Goats, Sheep, Gazelle, Antelope, and other Bovids
Sampling Instructions
If media not available, add 1-2 drops of sterile saline to the swab.
Bacterial culture swabs submitted in bacterial culture media (gel, charcoal, etc.) are NOT suitable for PCR testing.
Fresh samples provide the most accurate results.
Collection Container
Sealed, leak-proof container.
Packing Instructions
Label all samples. Ship overnight on cold packs in an insulated container.
Special Instructions
Sample types other than those listed above may be acceptable. A disclaimer will be included on the final report for non-validated species/specimen types. Contact the lab for more information.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Wednesday, Friday
Results available within 24 hours of test set up.
Vesicular stomatitis virus strain New Jersey & Indiana Serum Neutralization
Detection of antibodies to vesicular stomatitis virus (VSV) New Jersey (NJ) & Indiana (IND) strains by serum neutralization.
40160 Cattle, Equine VESICULAR STOMATITIS VIRUS INDIANA & NEW JERSEY VS VS SN VS NJ VS IND Horse vssn
Collect serum from clotted blood in a non-additive tube. Avoid hemolysis.
Collection Container
Serum samples should be submitted in serum separator tubes, uncoated/plain tubes may be appropriate for spun serum.
Packing Instructions
Label all samples with unique identifiers. Ship overnight on cold packs.
Special Instructions
Submit paired (acute & convalescent) serum samples together. Convalescent samples should be collected 10 to 14 days after the acute animal has been sampled. Separate acute serum from clot and store frozen until the convalescent sample has been collected. Comparison of sera from 2 points in time requires testing on the same day.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday
Results available Thursday
Virus Isolation- Bovine other
Isolation and identification of bovine viral diarrhea viurs (BVDV) from a follicle fluid, swab or tissue sample using cell culture and BVD PCR confirmation.
2-5 g fresh tissue, 2 ml fluid, swab in 5ml viral transport media
Species
Cattle
Sampling Instructions
Swabs must be submitted in viral transport media.
Collection Container
Fresh tissue, swabs or fluids must be submitted in sealed, leak proof containers.
Packing Instructions
Label all samples with unique identifiers. Ship overnight on cold packs.
Special Instructions
Turn-around time will be affected by a drastic increase in requests for an individual test, arrival time of the specimen at the laboratory and poor quality samples that will require additional processing.
Schedule and Location
Testing Lab
Testing Days
Normal Turnaround Time
WVDL - Madison
Monday, Wednesday, Friday
Results completed in 10-12 days
Virus Isolation- Non-Bovine
Isolation and identification of virus from a non-bovine fluid, swab or tissue sample using cell culture.